Inducible and Reversible Dominant-negative (DN) Protein InhibitionShikha Tarang 1, Umesh Pyakurel 1, Songila M.S.R. Doi 1, Michael D. Weston 1, Sonia M. Rocha-Sanchez 1
1Department of Oral Biology, Creighton University School of Dentistry
Here we present a protocol to develop a dominant-negative inducible system, in which any protein can be conditionally inactivated by reversibly overexpressing a dominant-negative mutant version of it.