In Vitro Testing of the TetO-DN-CB-myc6-Rb1 Transgene in NIH3T3 Cells
2:19
In Vitro Testing of the TetO-DN-CB-myc6-Rb1 Transgene in HEI-OC1 Cells
5:37
Generation of CBRb+/ROSA-CAG-rtTA+ (CBRb) Transgenic Mice and In Vivo Testing of the DN-CBRb Approach
6:09
Results: Confirmation of Efficiency of TetO-DN-CB-myc6-Rb1 Construct
7:50
Conclusion
Transcript
This method can help answer key questions in a wide variety of fields requiring conditional and transient inactivation of a gene of interest by reversibly over-expressing a dominant negative version of it. The main advantage of this technique is t
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Here we present a protocol to develop a dominant-negative inducible system, in which any protein can be conditionally inactivated by reversibly overexpressing a dominant-negative mutant version of it.