RIKEN Center for Biosystems Dynamics Research

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Recovery Cardiopulmonary Bypass Model Without Transfusion or Inotropic Agents in Rats

Shingo Hirao¹, Hidetoshi Masumoto¹, Tatsuya Itonaga¹, Kenji Minatoya¹

¹Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University

Here, we present a protocol to describe a simple recovery cardiopulmonary bypass model without transfusion or inotropic agents in a rat. This model allows the study of the long-term multiple organ sequelae of cardiopulmonary bypass.

JoVE Core

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Mari Mito^1,2, Mitsutaka Kadota³, Shinichi Nakagawa^1,4, Shintaro Iwasaki^2,5

¹RNA Biology Laboratory, RIKEN, ²RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, ³Laboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, ⁴RNA Biology Laboratory, Faculty of Pharmaceutical Sciences, Hokkaido University, ⁵Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo

We describe a step-by-step protocol for tandem chromatin immunoprecipitation sequencing (tChIP-Seq) that enables the analysis of cell-type-specific genome-wide histone modification.

Medicine

A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure

Hiroomi Nishio^1,3, Kenji Minatoya¹, Hidetoshi Masumoto^1,2

¹Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, ²Clinical Translational Research Program, RIKEN Center for Biosystems Dynamics Research, ³Department of Cardiovascular Surgery, Takamatsu Red Cross Hospital

The present protocol aims to experimentally create venous intimal hyperplasia by subjecting veins to arterial blood pressure for developing strategies to attenuate venous intimal hyperplasia following revascularization surgery using vein grafts.

Bioengineering

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Takeichiro Nakane^1,2,6, Mosha Abulaiti^1,2, Yuko Sasaki¹, William J. Kowalski³, Bradley B. Keller^4,5,7, Hidetoshi Masumoto^1,2

¹Clinical Translational Research Program, RIKEN Center for Biosystems Dynamics Research, ²Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, ³Laboratory of Stem Cell and Neuro-Vascular Biology, Cell and Developmental Biology Center, National Institutes of Health, ⁴Kosair Charities Pediatric Heart Research Program, Cardiovascular Innovation Institute, University of Louisville, ⁵Department of Pediatrics, School of Medicine, University of Louisville, ⁶Department of Cardiovascular Surgery, Mitsubishi Kyoto Hospital, ⁷Cincinnati Children's Heart Institute

The present protocol generates mesh-shaped engineered cardiac tissues containing cardiovascular cells derived from human induced pluripotent stem cells to allow the investigation of cell implantation therapy for heart diseases.

Biology

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Katsuyuki Nishi^1,2, Akiomi Nagasaka^1,2, Taro Sakamaki^1,2, Kay Sadaoka^1,2, Masanori Miyanishi^1,2

¹Hematopoietic Stem Cell Biology and Medical Innovation (HSCBMI), Department of Pediatrics, Kobe University Graduate School of Medicine, ²RIKEN Center for Biosystems Dynamics Research

We present a step-by-step protocol for the isolation of long-term hematopoietic stem cells (LT-HSCs) and short-term HSCs (ST-HSCs) using the Hoxb5 reporter system.