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Chinese PLA General Hospital

9 ARTICLES PUBLISHED IN JoVE

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Medicine

Digital Handwriting Analysis of Characters in Chinese Patients with Mild Cognitive Impairment
Hóngyi Zhào *1,2, Yanhai Zhang *3, Cuiqiao Xia 1, Yu Liu 2, Zhiyi Li 2, Yonghua Huang 1
1Department of Neurology, Chinese PLA General Hospital, 2Department of Neurology, NO 984 Hospital of PLA, 3Department of Cadre Ward, Xijing 986 Hospital, Fourth Military Medical University

This study proposed a digital handwriting analysis of characters in individuals with mild cognitive impairment to find more information than is revealed by traditional pencil–paper handwriting analysis.

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Genetics

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
Chengming Xu *1, Riqing Wei *2, Hui Lin 1, Leiyu Deng 1, Li Wang 3, Deyang Li 4, Honghui Den 5, Wensong Qin 1, Ping Wen 1, Ying Liu 1, Yingsong Wu 2, Qiang Ma 2, Jinliang Duan 1
1Centre for Women, Children, and Reproduction, Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 3Department of Obstetrics and Gynecology, Chinese PLA General Hospital, 4Clinical Laboratory, Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.

The protocol presents the overall in-lab procedures required in pre-implantation genetic testing for aneuploidy on a semiconductor-based next-generation sequencing platform. Here we present the detailed steps of whole genome amplification, DNA fragment selection, library construction, template preparation, and sequencing working flow with representative results.

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Biology

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model
Siting Zhang *1, Zeyu Cai *1, Xu Zhang 1, Tianfeng Ma 2, Wei Kong 1
1Department of Physiology and Pathophysiology, School of Basic Medical Sciences; Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University, 2Department of Vascular and Endovascular Surgery, the First Medical Centre, Chinese PLA General Hospital

This protocol describes a calcium phosphate-induced abdominal aortic aneurysm (AAA) mouse model to study the pathological features and molecular mechanisms of AAAs.

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Medicine

The Miniature Pig: A Large Animal Model for Cochlear Implant Research
Xiaojun Ji *1,2,3,4, Yi Luo *1,2,3,4, Weiwei Guo 1,2,3,4, Fei Ji 1,2,3,4, Shuolong Yuan 1,2,3,4, Liangwei Xu 1,2,3,4, Wei Chen *1,2,3,4
1College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, 2National Clinical Research Center for Otolaryngologic Diseases, 3Key Lab of Hearing Science, Ministry of Education, 4Beijing Key Lab of Hearing Impairment for Prevention and Treatment

Miniature pigs (mini-pigs) are an ideal large animal model for research into cochlear implants. Cochlear implantation surgery in mini-pigs can be utilized to provide initial evidence of the safety and potential performance of novel electrode arrays and surgical approaches in a living system similar to human beings.

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Medicine

A Model of Long-Term Ventricular Fibrillation in Isolated Rat Hearts
Xiaoyi He 1,2, Libing Li 1, Wenhong Xu 1, Shengli Jiang 1,2
1Cardiovascular Surgery, First Medical Center, Chinese PLA General Hospital, 2Medical School of Chinese PLA

This protocol presents a model of long-term ventricular fibrillation in rat hearts induced by continuous stimulation with low-voltage alternating current. This model has a high success rate, is stable, reliable, and reproducible, has a low impact on cardiac function, and causes only mild myocardial injury.

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Medicine

Mouse Wound Models and Preparation of Single-Cell Suspensions
Yuanyuan Liu 1, Mingwang Zhang 2, Kai Luo 3, Yurui Zou 4, Rongya Yang 5
1Medical School of Chinese People's Liberation Army, 2Department of Dermatology, Southwest Hospital, Army Medical University, 3Biomedical Treatment Center, the Seventh Medical Center, Chinese PLA General Hospital, 4The 83th Affiliated Hospital, Xinxiang Medical University, 5Department of Dermatology, the Seventh Medical Center, Chinese PLA General Hospital

This study presents two crucial experimental techniques: the construction of a murine wound model for assessing wound closure and the preparation of single-cell suspensions from murine skin to preserve cellular viability.

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Medicine

Purification and Characterization of Extracellular Vesicles from Human Adipose-Derived Mesenchymal Stem Cells
Yuting Wang 1,2, Yan Han 1, Yudi Han 1
1Department of Plastic and Reconstructive Surgery, the First Medical Centre, Chinese PLA General Hospital, 2School of Medicine, Nankai University

This protocol describes all procedures, from culturing human adipose-derived mesenchymal stem cells (ADSCs) and collecting supernatant to extracting extracellular vesicles (EVs) using ultracentrifugation.

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Medicine

Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells
Tianqi Li 1,2,3, Xiangbo Meng 1,2, Shuaichen Li 1,2, Sunxin Zhou 1,2, Hongkun Li 1,2, Shi Quan 2, Tong Zhang 2
1Medical School of Chinese PLA, 2Department of Stomatology, the First Medical Centre, Chinese PLA General Hospital, 3Department of Stomatology, Fujian People's Armed Police Corps Hospital

Mesenchymal stem cells from the jaw bone marrow have significant functions in diverse differentiation, self-renewal, and immune modulation. They have emerged as a crucial reservoir of precursor cells in gene therapy, tissue engineering, and regenerative medicine. Here, we present a unique method for isolating jaw bone marrow mesenchymal stem cells in rats.

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Bioengineering

A Medical Decision Support Platform for Identifying Thrombospondin 1 in Non-alcoholic Fatty Liver Disease via Immuno-Infiltration Analysis
Jianyu Huang 1,2,3, Pengfei Li 1,4, Jianing Chi 1,4, Jiaman Hu 1, Hua Cai 1, Ningxia Wu 1, Min Li 1, Zhongqiu Lin 1, Yutong Ji 1, Jiajia Xu 1, Peng Liu 5, Senthil Kumar Jagatheesaperumal 6, Victor Hugo C. de Albuquerque 7, Lin Xu 1,2,3,4
1Department of Geriatric Cardiology, General Hospital of Southern Theater Command, 2Guangzhou Key Laboratory of Cardiac Rehabilitation, 3Branch of National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, 4The First School of Clinical Medicine, Southern Medical University, 5Zhujiang Hospital, Southern Medical University, Cardiovascular Department/The Second School of Clinical Medicine, Southern Medical University, 6Department of Electronics & Communication Engineering, Mepco Schlenk Engineering College, 7Department of Teleinformatics Engineering, Federal University of Ceará

This study investigated the relationships between non-alcoholic fatty liver disease (NAFLD) and myocardial infarction (MI) through co-expressed genes, identifying Thrombospondin 1 (THBS1) as a biomarker. Immuno-infiltration analysis revealed CD8+ T cells and neutrophils as key factors, with THBS1 showing potential as a diagnostic tool for NAFLD and MI.

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