UNIVERSITY HOSPITALS CLEVELAND MEDICAL CENTER

3 ARTICLES PUBLISHED IN JoVE

Medicine

Ileectomy-induced Bile Overaccumulation in Mouse Intestine

Rongli Zhang^*1,2,3, Jonathan W. Ray^1,2, Mukesh K. Jain^1,2, Shuxin Han^*1,2

¹Department of Medicine, Case Cardiovascular Research Institute, Case Western Reserve University, ²Harrington Heart and Vascular Institute, University Hospitals Cleveland Medical Center, ³Institute for Transformative Molecular Medicine, Case Western Reserve University School of Medicine, University Hospitals Cleveland Medical Center

Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.

Bioengineering

Optical Imaging of Isolated Murine Ventricular Myocytes

Shuxin Han^1,2, Matt Klos³, Sherry Morgenstern³, Ramiz Ahmad³, Isabella Pua³, Shreyas Suresh¹, Kayla Hicks³, Eric Devaney³

¹Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China, ²Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, ³Pediatric Cardiac and Thoracic Surgery, University Hospitals Cleveland Medical Center

We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements.

Biology

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Dionysios C. Watson^1,2,3, Sadie Johnson¹, Akeem Santos^1,4, Mei Yin⁵, Defne Bayik¹, Justin D. Lathia^1,3, Mohammed Dwidar^1,3,4

¹Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, ²University Hospitals Cleveland Medical Center, ³Case Western Reserve University, ⁴Center for Microbiome & Human Health, Cleveland Clinic, ⁵Electron Microscopy Core, Lerner Research Institute, Cleveland Clinic

Bacteria secrete nanometer-sized extracellular vesicles (EVs) carrying bioactive biological molecules. EV research focuses on understanding their biogenesis, role in microbe-microbe and host-microbe interactions and disease, as well as their potential therapeutic applications. A workflow for scalable isolation of EVs from various bacteria is presented to facilitate standardization of EV research.