Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

3.6K Views

•

09:56 min

•

October 13th, 2021

DOI :

10.3791/63155-v

October 13th, 2021

•

Dionysios C. Watson¹^,²^,³, Sadie Johnson¹, Akeem Santos¹^,⁴, Mei Yin⁵, Defne Bayik¹, Justin D. Lathia¹^,³, Mohammed Dwidar¹^,³^,⁴

¹Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, ²University Hospitals Cleveland Medical Center, ³Case Western Reserve University, ⁴Center for Microbiome & Human Health, Cleveland Clinic, ⁵Electron Microscopy Core, Lerner Research Institute, Cleveland Clinic

Summary

Explore More Videos

Extracellular Vesicles

Escherichia Coli

Bacterial Cultures

Scalable Isolation

Purification Protocol

In Vivo Studies

Therapeutic Manufacturing

Chapters in this video

0:05

Introduction

1:09

Culturing Conditions and Extracellular Vesicles Isolation

5:15

Extracellular Vesicles (EVs) Isolation with Size Exclusion Chromatography (SEC)

7:15

EV Preparation Quality Control and Storage

7:58

Results: Analysis of EVs Fraction Collected from Escherichia coli MP1

9:24

Conclusion

Related Videos

09:49

Isolation and Purification of Kinesin from Drosophila Embryos

12.1K Views

14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

48.2K Views

07:10

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

76.7K Views

07:35

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

21.6K Views

09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

16.3K Views

08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

13.6K Views

09:30

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

7.4K Views

08:44

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

5.4K Views

08:03

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

2.5K Views

09:57

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

1.9K Views