Efficient Retroviral Transduction and Competitive Homing for Investigating GPCR-Mediated T-Cell Localization in Diverse Tissue MicroenvironmentsAiman Ayesha 1,2,3, Borja Ocon 1,2,3, Yuhan Bi 1,2,3, Eugene C. Butcher 1,2,3
1Department of Pathology, Stanford University School of Medicine, 2Palo Alto Veterans Institute for Research, 3Veterans Affairs Palo Alto Health Care System
We present improved protocols for retroviral transduction of trafficking receptors and competitive homing to study receptor-mediated organ- and microenvironment-specific lymphocyte positioning. This method offers valuable insights into immune cell trafficking mechanisms and has potential applications in future basic and therapeutic research.
