Palo Alto Veterans Institute for Research

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Efficient Retroviral Transduction and Competitive Homing for Investigating GPCR-Mediated T-Cell Localization in Diverse Tissue Microenvironments

Aiman Ayesha^1,2,3, Borja Ocon^1,2,3, Yuhan Bi^1,2,3, Eugene C. Butcher^1,2,3

¹Department of Pathology, Stanford University School of Medicine, ²Palo Alto Veterans Institute for Research, ³Veterans Affairs Palo Alto Health Care System

We present improved protocols for retroviral transduction of trafficking receptors and competitive homing to study receptor-mediated organ- and microenvironment-specific lymphocyte positioning. This method offers valuable insights into immune cell trafficking mechanisms and has potential applications in future basic and therapeutic research.