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Soroka Medical Center, Ben-Gurion University of the Negev

9 ARTICLES PUBLISHED IN JoVE

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Behavior

A New Method for Inducing a Depression-Like Behavior in Rats
Vladimir Zeldetz *1, Dmitry Natanel *2, Matthew Boyko 2, Alexander Zlotnik 2, Honore N. Shiyntum 3, Julia Grinshpun 2, Dmitry Frank 2, Ruslan Kuts 2, Evgeni Brotfain 2, Jochanan Peiser 2
1Department of Emergent Medicine, Soroka University Medical Center, Ben-Gurion University of the Negev, 2Division of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, 3Department of Biophysics and Biochemistry, Oles' Honchar Dnipro National University

This protocol describes a new model by which healthy rats could contract depression over a given time periodthrough contagion by exposure to chronic unpredictable stressed (CUS) rats.

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Behavior

A Middle Cerebral Artery Occlusion Technique for Inducing Post-stroke Depression in Rats
Ruslan Kuts *1, Israel Melamed *2, Honore N. Shiyntum 3, Dmitry Frank 1, Julia Grinshpun 1, Alexander Zlotnik 1, Evgeni Brotfain 1, Michael Dubilet 1, Dmitry Natanel 1, Matthew Boyko 1
1Department of Anesthesiology and Critical Care, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2Department of Neurosurgery, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Department of Biophysics and Biochemistry, Faculty of Biology, Ecology, and Medicine, Oles Honchar Dnipro National University

Here we present a protocol to induce post-stroke depression in rats by occluding the middle cerebral artery via the internal carotid artery. We use the Porsolt forced swim test and the sucrose preference test to confirm and evaluate induced depressive moods.

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Medicine

Inducing Acute Liver Injury in Rats via Carbon Tetrachloride (CCl4) Exposure Through an Orogastric Tube
Dmitry Frank *1, Shiri Savir *1, Benjamin F. Gruenbaum 2, Israel Melamed 3, Julia Grinshpun 1, Ruslan Kuts 1, Boris Knyazer 4, Alexander Zlotnik 1, Max Vinokur 1, Matthew Boyko 1
1Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, 2Department of Anesthesiology, Yale University School of Medicine, 3Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 4Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

This protocol describes a common and feasible method of inducing acute liver injury (ALI) via CCl4 exposure through an orogastric tube. CCl4 exposure induces ALI through the formation of reactive oxygen species during its biotransformation in the liver. This method is used to analyze the pathophysiology of ALI and examine different hepatoprotective strategies.

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Neuroscience

Laser-Induced Brain Injury in the Motor Cortex of Rats
Ruslan Kuts *1, Israel Melamed *2, Honore N. Shiyntum 3, Benjamin F. Gruenbaum 4, Dmitry Frank 1, Boris Knyazer 5, Dmitry Natanel 1, Olena Severynovska 3, Max Vinokur 1, Matthew Boyko 1
1Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 2Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Department of Biochemistry and Physiology, Faculty of Biology, Ecology, and Medicine, Oles Honchar Dnipro National University, 4Department of Anesthesiology, Yale University School of Medicine, 5Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

The protocol presented here shows a technique to create a rodent model of brain injury. The method described here uses laser irradiation and targets motor cortex.

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Neuroscience

Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration
Dmitry Frank *1, Israel Melamed *2, Benjamin F. Gruenbaum 3, Julia Grinshpun 1, Ruslan Kuts 1, Rachel Shvartsur 4, Abed N. Azab 4, Mohamad H. Assadi 5, Max Vinokur 1, Matthew Boyko 1
1Division of Anesthesia and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 2Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Department of Anesthesiology, Yale University School of Medicine, 4Recanati School for Community Health Professions, Faculty of Health Sciences, Ben-Gurion University of the Negev, 5Department of microbiology and immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev

This protocol validates a reliable, easy-to-perform and reproducible rodent model of brain diffuse axonal injury (DAI) that induces widespread white matter damage without skull fractures or contusions.

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Neuroscience

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples
Dmitry Frank *1, Benjamin F. Gruenbaum *2, Julia Grinshpun 1, Israel Melamed 3, Olena Severynovska 4, Ruslan Kuts 1, Michael Semyonov 1, Evgeni Brotfain 1, Alexander Zlotnik 1, Matthew Boyko 1
1Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, 2Department of Anesthesiology, Yale University School of Medicine, 3Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 4Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University

This protocol describes a novel technique of measuring the three most important parameters of ischemic brain injury on the same set of rodent brain samples. Using only one brain sample is highly advantageous in terms of ethical and economic costs.

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Behavior

A Complex Diving-For-Food Task to Investigate Social Organization and Interactions in Rats
Benjamin F. Gruenbaum *1, Dmitry Frank *2, Shiri Savir 2, Honore N. Shiyntum 3, Ruslan Kuts 2, Max Vinokur 2, Israel Melamed 4, Michael Dubilet 2, Alexander Zlotnik 2, Matthew Boyko 2
1Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, 2Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Department of Biophysics and Biochemistry, Oles Honchar Dnipro National University, 4Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

This protocol describes a method of examining social hierarchy in a rat model. Rats perform a complex diving-for-food task in which they form a distinct hierarchy according to their willingness to dive underwater and swim to obtain a food pellet. This method is used to understand decision making and social relationships among highly social animals in small groups.

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Neuroscience

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury
Dmitry Frank *1, Benjamin F. Gruenbaum *2, Israel Melamed 3, Julia Grinshpun 1, Yair Benjamin 1, Ievgeni Vzhetson 1, Nadia Kravchenko 4, Michael Dubilet 1, Matthew Boyko *1, Alexander Zlotnik *1
1Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, 3Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 4Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University

Traumatic brain injury (TBI) is commonly associated with memory impairment. Here, we present a protocol to assess spatial working memory after TBI via a metric task. A metric test is a useful tool to study spatial working memory impairment after TBI.

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Behavior

Assessing Dominant-Submissive Behavior in Adult Rats Following Traumatic Brain Injury
Dmitry Frank *1, Benjamin F. Gruenbaum *2, Michael Semyonov 1, Yair Binyamin 1, Olena Severynovska 3, Ron Gal 1, Amit Frenkel 1, Boris Knazer 4, Matthew Boyko 1, Alexander Zlotnik 1
1Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, 3Department of Biochemistry and Physiology of the Faculty of Biology and Ecology, Oles Gonchar of the Dnipro National University, 4Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

The present protocol describes a rat model of fluid percussion-induced traumatic brain injury followed by a series of behavioral tests to understand the development of dominant and submissive behavior. Using this model of traumatic brain injury in conjunction with specific behavioral tests enables the study of social impairments following brain injury.

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