Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

7.8K Views

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04:32 min

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October 23rd, 2020

DOI :

10.3791/61309-v

October 23rd, 2020

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Dmitry Frank*¹, Benjamin F. Gruenbaum*², Julia Grinshpun¹, Israel Melamed³, Olena Severynovska⁴, Ruslan Kuts¹, Michael Semyonov¹, Evgeni Brotfain¹, Alexander Zlotnik¹, Matthew Boyko¹

¹Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, ²Department of Anesthesiology, Yale University School of Medicine, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ⁴Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University

Summary

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Ischemic Brain Injury

Rodent Brain Samples

Middle Cerebral Artery Occlusion

Luminescence Spectrophotometry

Chapters in this video

0:04

Introduction

0:54

Determination of Infarct Zone and Brain Edema

2:37

Determination of BBB Disruption

3:23

Results: Histological Outcomes of MCAO Rats Compared to Sham-operated Rats

3:57

Conclusion

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