Soroka Medical Center, Ben-Gurion University of the Negev

9 ARTICLES PUBLISHED IN JoVE

Behavior

A New Method for Inducing a Depression-Like Behavior in Rats

Vladimir Zeldetz^*1, Dmitry Natanel^*2, Matthew Boyko², Alexander Zlotnik², Honore N. Shiyntum³, Julia Grinshpun², Dmitry Frank², Ruslan Kuts², Evgeni Brotfain², Jochanan Peiser²

¹Department of Emergent Medicine, Soroka University Medical Center, Ben-Gurion University of the Negev, ²Division of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, ³Department of Biophysics and Biochemistry, Oles' Honchar Dnipro National University

This protocol describes a new model by which healthy rats could contract depression over a given time periodthrough contagion by exposure to chronic unpredictable stressed (CUS) rats.

Behavior

A Middle Cerebral Artery Occlusion Technique for Inducing Post-stroke Depression in Rats

Ruslan Kuts^*1, Israel Melamed^*2, Honore N. Shiyntum³, Dmitry Frank¹, Julia Grinshpun¹, Alexander Zlotnik¹, Evgeni Brotfain¹, Michael Dubilet¹, Dmitry Natanel¹, Matthew Boyko¹

¹Department of Anesthesiology and Critical Care, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, ²Department of Neurosurgery, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, ³Department of Biophysics and Biochemistry, Faculty of Biology, Ecology, and Medicine, Oles Honchar Dnipro National University

Here we present a protocol to induce post-stroke depression in rats by occluding the middle cerebral artery via the internal carotid artery. We use the Porsolt forced swim test and the sucrose preference test to confirm and evaluate induced depressive moods.

Medicine

Inducing Acute Liver Injury in Rats via Carbon Tetrachloride (CCl₄) Exposure Through an Orogastric Tube

Dmitry Frank^*1, Shiri Savir^*1, Benjamin F. Gruenbaum², Israel Melamed³, Julia Grinshpun¹, Ruslan Kuts¹, Boris Knyazer⁴, Alexander Zlotnik¹, Max Vinokur¹, Matthew Boyko¹

¹Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, ²Department of Anesthesiology, Yale University School of Medicine, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ⁴Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

This protocol describes a common and feasible method of inducing acute liver injury (ALI) via CCl₄ exposure through an orogastric tube. CCl₄ exposure induces ALI through the formation of reactive oxygen species during its biotransformation in the liver. This method is used to analyze the pathophysiology of ALI and examine different hepatoprotective strategies.

Neuroscience

Laser-Induced Brain Injury in the Motor Cortex of Rats

Ruslan Kuts^*1, Israel Melamed^*2, Honore N. Shiyntum³, Benjamin F. Gruenbaum⁴, Dmitry Frank¹, Boris Knyazer⁵, Dmitry Natanel¹, Olena Severynovska³, Max Vinokur¹, Matthew Boyko¹

¹Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ²Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ³Department of Biochemistry and Physiology, Faculty of Biology, Ecology, and Medicine, Oles Honchar Dnipro National University, ⁴Department of Anesthesiology, Yale University School of Medicine, ⁵Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

The protocol presented here shows a technique to create a rodent model of brain injury. The method described here uses laser irradiation and targets motor cortex.

Neuroscience

Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration

Dmitry Frank^*1, Israel Melamed^*2, Benjamin F. Gruenbaum³, Julia Grinshpun¹, Ruslan Kuts¹, Rachel Shvartsur⁴, Abed N. Azab⁴, Mohamad H. Assadi⁵, Max Vinokur¹, Matthew Boyko¹

¹Division of Anesthesia and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ²Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ³Department of Anesthesiology, Yale University School of Medicine, ⁴Recanati School for Community Health Professions, Faculty of Health Sciences, Ben-Gurion University of the Negev, ⁵Department of microbiology and immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev

This protocol validates a reliable, easy-to-perform and reproducible rodent model of brain diffuse axonal injury (DAI) that induces widespread white matter damage without skull fractures or contusions.

Neuroscience

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

Dmitry Frank^*1, Benjamin F. Gruenbaum^*2, Julia Grinshpun¹, Israel Melamed³, Olena Severynovska⁴, Ruslan Kuts¹, Michael Semyonov¹, Evgeni Brotfain¹, Alexander Zlotnik¹, Matthew Boyko¹

¹Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, ²Department of Anesthesiology, Yale University School of Medicine, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ⁴Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University

This protocol describes a novel technique of measuring the three most important parameters of ischemic brain injury on the same set of rodent brain samples. Using only one brain sample is highly advantageous in terms of ethical and economic costs.

Behavior

A Complex Diving-For-Food Task to Investigate Social Organization and Interactions in Rats

Benjamin F. Gruenbaum^*1, Dmitry Frank^*2, Shiri Savir², Honore N. Shiyntum³, Ruslan Kuts², Max Vinokur², Israel Melamed⁴, Michael Dubilet², Alexander Zlotnik², Matthew Boyko²

¹Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, ²Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ³Department of Biophysics and Biochemistry, Oles Honchar Dnipro National University, ⁴Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

This protocol describes a method of examining social hierarchy in a rat model. Rats perform a complex diving-for-food task in which they form a distinct hierarchy according to their willingness to dive underwater and swim to obtain a food pellet. This method is used to understand decision making and social relationships among highly social animals in small groups.

Neuroscience

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury

Dmitry Frank^*1, Benjamin F. Gruenbaum^*2, Israel Melamed³, Julia Grinshpun¹, Yair Benjamin¹, Ievgeni Vzhetson¹, Nadia Kravchenko⁴, Michael Dubilet¹, Matthew Boyko^*1, Alexander Zlotnik^*1

¹Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, ²Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ⁴Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University

Traumatic brain injury (TBI) is commonly associated with memory impairment. Here, we present a protocol to assess spatial working memory after TBI via a metric task. A metric test is a useful tool to study spatial working memory impairment after TBI.

Behavior

Assessing Dominant-Submissive Behavior in Adult Rats Following Traumatic Brain Injury

Dmitry Frank^*1, Benjamin F. Gruenbaum^*2, Michael Semyonov¹, Yair Binyamin¹, Olena Severynovska³, Ron Gal¹, Amit Frenkel¹, Boris Knazer⁴, Matthew Boyko¹, Alexander Zlotnik¹

¹Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, ²Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, ³Department of Biochemistry and Physiology of the Faculty of Biology and Ecology, Oles Gonchar of the Dnipro National University, ⁴Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

The present protocol describes a rat model of fluid percussion-induced traumatic brain injury followed by a series of behavioral tests to understand the development of dominant and submissive behavior. Using this model of traumatic brain injury in conjunction with specific behavioral tests enables the study of social impairments following brain injury.