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La Jolla Institute for Immunology

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
Yanfang Peipei Zhu 1, Lindsey Padgett 1, Huy Q. Dinh 1, Paola Marcovecchio 1, Runpei Wu 1, Denise Hinz 2, Cheryl Kim 2, Catherine C. Hedrick 1
1Division of Inflammation Biology, La Jolla Institute for Immunology, 2Flow Cytometry Core Facility, La Jolla Institute for Immunology

Here, we present a protocol to process fresh bone marrow (BM) isolated from mouse or human for high-dimensional mass cytometry (Cytometry by Time-Of-Flight, CyTOF) analysis of neutrophil-lineage cells.

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Biology

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
Justin Cayford 1, Sara Herrera-de la Mata 1, Benjamin Joachim Schmiedel 1, Vivek Chandra 1, Pandurangan Vijayanad 1, Grégory Seumois 1
1Division of Vaccine Discovery, La Jolla Institute for Immunology

This paper describes a semiautomated, microscaled ChIP-Seq protocol of DNA regions associated with a specific histone modification (H3K27ac) using a ChIP liquid-handler platform, followed by library preparation using tagmentation. The procedure includes control assessment for quality and quantity purposes and can be adopted to other histone modifications or transcription factors.

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JoVE Core

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
Hsin Yu Liu 1, Amnon Altman 1, Ann J. Canonigo-Balancio 1, Michael Croft 1
1La Jolla Institute for Immunology

The protocol presents a cancer immunotherapy model using cell-based tumor vaccination with Flt3L-expressing B16-F10 melanoma. This protocol demonstrates the procedures, including preparation of cultured tumor cells, tumor implantation, cell irradiation, measurement of tumor growth, isolation of intratumoral immune cells, and flow cytometry analysis.

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