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A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

DOI :

10.3791/61617-v

8:04 min

August 13th, 2020

August 13th, 2020

2,619 Views

1Division of Vaccine Discovery, La Jolla Institute for Immunology

This paper describes a semiautomated, microscaled ChIP-Seq protocol of DNA regions associated with a specific histone modification (H3K27ac) using a ChIP liquid-handler platform, followed by library preparation using tagmentation. The procedure includes control assessment for quality and quantity purposes and can be adopted to other histone modifications or transcription factors.

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Semiautomated ChIP Seq

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