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Faculdade de Ciências da Universidade de Lisboa

3 ARTICLES PUBLISHED IN JoVE

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Environment

BtM, a Low-cost Open-source Datalogger to Estimate the Water Content of Nonvascular Cryptogams
María Leo *1, Angel Lareo *2, Carlos Garcia-Saura 2, Joaquín Hortal 3,4, Nagore G. Medina 5
1Real Jardín Botánico (CSIC-RJB), 2Grupo de Neurocomputación Biológica, Dpto. de Ingeniería Informática, Escuela Politécnica Superior, Universidad Autónoma de Madrid, 3Department of Biogeography and Global Change, Museo Nacional de Ciencias Naturales (MNCN-CSIC), 4Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências da Universidade de Lisboa, 5Department of Botany, Faculty of Science, University of South Bohemia

We present a simple and cost-effective method to build an open-source datalogger that measures the conductance of nonvascular cryptogams together with the environmental temperature and humidity. We describe the hardware design of the datalogger and provide step-by-step assembly instructions, the list of required open-source logging software, the code to run the datalogger, and a calibration protocol.

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Developmental Biology

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
André Dias 1, Gabriel G. Martins 1,2, Alexandre Lopes 1,3, Moisés Mallo 1
1Instituto Gulbenkian de Ciência, 2Faculdade de Ciências da Universidade de Lisboa, 3NOVA School of Science and Technology

Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.

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Biology

Infection of In Vivo and In Vitro Pines with the Pinewood Nematode Bursaphelenchus xylophilus and Isolation of Induced Volatiles
Jorge M. S. Faria 1,2, A. Cristina Figueiredo 3,4, Dora M. Teixeira 5, Maria L. Inácio 1,2
1INIAV, I.P., National Institute for Agrarian and Veterinary Research, I.P., 2GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB NOVA), 3Centre for Environmental and Marine Studies (CESAM Lisboa), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, 4Centre for Ecology, Evolution and Environmental Changes (CE3C), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, 5Hercules Laboratory & Chemistry and Biochemistry Department of Science and Technology School, University of Évora

The protocol describes the in vivo and in vitro pinewood nematode infection of Pinus pinaster and their volatilome analysis through Gas Chromatography (GC) and GC coupled to Mass Spectrometry (GC-MS).

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