Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.
The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved