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Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

DOI :

10.3791/64196-v

August 4th, 2022

August 4th, 2022

1,498 Views

1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology

The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.

Tags

Microfluidics assisted

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