Staining with Mitochondrial Membrane Potential Sensitive Dye
4:14
Live‐Cell Imaging
5:33
Results: Mitochondrial Staining in Primary Hippocampal Neurons
6:10
Conclusion
Transcript
Mitochondria are essential for neuronal health. In many neurodegenerative diseases, axonal mitochondria are the first ones to suffer, but it is not clear what makes axonal mitochondria so special. The fluidic pressure gradient in the chambers used
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The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.