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University of Wisconsin-Madison School of Medicine and Public Health

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
Di Lang 1, Matthew Sulkin 1, Qing Lou 1, Igor R. Efimov 1
1Department of Biomedical Engineering, Washington University in St. Louis

This paper details the dissection procedure, instrumental setup, and experimental conditions during optical mapping of transmembrane potential (Vm) and intracellular calcium transient (CaT) in intact isolated Langendorff perfused mouse hearts.

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Biology

High-resolution Optical Mapping of the Mouse Sino-atrial Node
Di Lang 1, Alexey V. Glukhov 1
1Department of Medicine, University of Wisconsin-Madison School of Medicine and Public Health

Here, we present a protocol for optical mapping of electrical activity from the mouse right atrium and especially the sino-atrial node, at a high spatial and temporal resolution.

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Cancer Research

Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad
Lauren B. Shunkwiler 1, Jill D. Haag 2, Michael N. Gould 2, Bart M. G. Smits 1
1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 2Department of Oncology, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison School of Medicine and Public Health

This article describes a transplantation method to graft donor rat mammary epithelial cells into the interscapular white fat pad of recipient animals. This method can be used to examine host and/or donor effects on mammary epithelium development and eliminates the need for pre-clearing, thereby extending the usefulness of this technique.

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Developmental Biology

Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
Rebecca J. Salamon 1, Ziheng Zhang 1, Ahmed I. Mahmoud 1
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health

Cardiomyocyte proliferation following injury is a dynamic process that requires a symphony of extracellular cues from non-myocyte cell populations. Utilizing lineage tracing, passive CLARITY, and three-dimensional whole-mount confocal microscopy techniques, we can analyze the influence of a variety of cell types on cardiac repair and regeneration.

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Biology

Advanced Abdominal Aortic Aneurysm Modeling in Mice by Combination of Topical Elastase and Oral ß-aminopropionitrile
Jack Bontekoe 1, Gilbert Upchurch 2, Courtney Morgan 1, Bo Liu 3
1Division of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison School of Medicine and Public Health, 2Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine, 3Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health

This protocol describes a methodical surgical approach to modeling advanced abdominal aortic aneurysms in mice by a combination of directly applying elastase to the infrarenal aorta and administering ß-aminopropionitrile through drinking water.

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