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Shenzhen Bay laboratory

7 ARTICLES PUBLISHED IN JoVE

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Engineering

Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices
Yan Wu 1, Xiang Qian 1, Shengli Mi 1, Min Zhang 1, Shuqing Sun 1, Xiaohao Wang 1,2
1Graduate School at Shenzhen, Tsinghua University, 2The State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University

Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.

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Chemistry

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
Xiaodong Shi *1, Yinghuan Liu *1, Rongtong Zhao 1, Zigang Li 1
1Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School

We present a protocol for the construction of thioether/vinyl sulfide-tethered helical peptides using photo-induced thiol-ene/thiol-yne hydrothiolation.

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JoVE Core

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
Lingxiao Chaihu 1,2, Xiaopeng Yao 1, Xiang Xu 1,2, Zhongqin Zhu 1, David D. Y. Chen 3, Guanbo Wang 1
1School of Chemistry and Materials Science, Nanjing Normal University, 2Institute for Cell Analysis, Shenzhen Bay Laboratory, 3Department of Chemistry, University of British Columbia

Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation.

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Behavior

Individualized rTMS Treatment for Depression using an fMRI-Based Targeting Method
Xin Luo 1, Yiru Hu 1, Runhua Wang 1, Min Zhang 1, Xiaomei Zhong 1, Bin Zhang 1
1Affiliated Brain Hospital of Guangzhou Medical University

The present protocol describes the application of repetitive transcranial magnetic stimulation (rTMS), where a subregion of the dorsolateral prefrontal cortex (DLPFC) with the strongest functional anticorrelation with the subgenual anterior cingulate cortex (sgACC) was located as the stimulation target under the assistance of a fMRI-based neuronavigation system.

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Biology

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
Qiang Yan *1, Yingying He *2,3, Yang Yue 2,3, Luyao Jie 1, Tingmei Wen 2,3, Yumiao Zhao 2,3, Min Zhang 2, Tingting Zhang 2
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 2Research Institute of Applied Biology, Shanxi University, 3School of Life Science, Shanxi University

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

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Chemistry

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Chunli Song *1, Zhanfeng Hou *1,2, Zijun Jiao *1, Zhaodi Liu 3, Chenshan Lian 1,2, Min Zhang 1, Wei Liang 3, Feng Yin 1,2, Zigang Li 1,2
1Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, 2Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 3Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University

This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.

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Cancer Research

Spontaneous Murine Model of Anaplastic Thyroid Cancer
Huayun Yan 1, Yingfang Ma 1, Xinyue Zhou 2, Yushuang He 3, Yang Liu 2, Carlos Caulin 4, Leiming Wang 5, Heng Xu 1,6, Han Luo 2,6
1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, 2Division of Thyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-Related Molecular Network, Department of General Surgery, West China Hospital, Sichuan University, 3Department of Ultrasound, West China Hospital of Sichuan University, Sichuan University, 4Department of Otolaryngology-Head & Neck Surgery and University of Arizona Cancer Center, University of Arizona, 5Center for Translational Medicine, Shenzhen Bay Laboratory, 6Division of Laboratory Medicine/Research Centre of Clinical Laboratory Medicine, West China Hospital, Sichuan University

Here, we present a standard pipeline to obtain murine ATC tumors by spontaneous genetically engineered mouse models. Further, we present tumor dynamics and pathological information about the primary and metastasized lesions. This model will help researchers to understand tumorigenesis and facilitate drug discoveries.

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