Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

3.2K Views

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05:45 min

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June 8th, 2021

DOI :

10.3791/62672-v

June 8th, 2021

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Lingxiao Chaihu¹^,², Xiaopeng Yao¹, Xiang Xu¹^,², Zhongqin Zhu¹, David D. Y. Chen³, Guanbo Wang¹

¹School of Chemistry and Materials Science, Nanjing Normal University, ²Institute for Cell Analysis, Shenzhen Bay Laboratory, ³Department of Chemistry, University of British Columbia

Summary

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Capillary Electrophoresis

Hydrogen Deuterium Exchange

Protein Conformational Characterization

Top down Mass Spectrometry

Electrophoretic Effect

Coexisting Protein Species

HDX Reactions

Microvial CE MS Interface

Background Electrolyte Solution

Nano electrospray Ionization

Infusion Pump

Electrophoretic Voltage

Electropherogram Acquisition

Chapters in this video

0:04

Introduction

0:38

Operation of CE-Based HDX MS Analysis

4:01

Results: Conformational Characterization of Proteins with Top-Down Mass Spectrometry

5:18

Conclusion

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