JoVE Logo

Sign In

Affiliated Brain Hospital of Guangzhou Medical University

5 ARTICLES PUBLISHED IN JoVE

image

Engineering

Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices
Yan Wu 1, Xiang Qian 1, Shengli Mi 1, Min Zhang 1, Shuqing Sun 1, Xiaohao Wang 1,2
1Graduate School at Shenzhen, Tsinghua University, 2The State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University

Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.

image

Environment

Studying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae
Bin Zhang 1,2, Xinyue Yang 2, Jing Zhao 3, Ting Xu 2,4, Daqiang Yin 2,4
1State Key Laboratory of Marine Geology, Tongji University, 2Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, 3Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Center of 10 Shanghai Polytechnic University, 4Shanghai Institute of Pollution Control and Ecological Security

A detailed experimental protocol is presented in this paper for the evaluation of neurobehavioral toxicity of environmental pollutants using a zebrafish larvae model, including the exposure process and tests for neurobehavioral indicators.

image

Behavior

Individualized rTMS Treatment for Depression using an fMRI-Based Targeting Method
Xin Luo 1, Yiru Hu 1, Runhua Wang 1, Min Zhang 1, Xiaomei Zhong 1, Bin Zhang 1
1Affiliated Brain Hospital of Guangzhou Medical University

The present protocol describes the application of repetitive transcranial magnetic stimulation (rTMS), where a subregion of the dorsolateral prefrontal cortex (DLPFC) with the strongest functional anticorrelation with the subgenual anterior cingulate cortex (sgACC) was located as the stimulation target under the assistance of a fMRI-based neuronavigation system.

image

Biology

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
Qiang Yan *1, Yingying He *2,3, Yang Yue 2,3, Luyao Jie 1, Tingmei Wen 2,3, Yumiao Zhao 2,3, Min Zhang 2, Tingting Zhang 2
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 2Research Institute of Applied Biology, Shanxi University, 3School of Life Science, Shanxi University

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

image

Chemistry

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Chunli Song *1, Zhanfeng Hou *1,2, Zijun Jiao *1, Zhaodi Liu 3, Chenshan Lian 1,2, Min Zhang 1, Wei Liang 3, Feng Yin 1,2, Zigang Li 1,2
1Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, 2Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 3Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University

This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved