Affiliated Brain Hospital of Guangzhou Medical University

5 ARTICLES PUBLISHED IN JoVE

Engineering

Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices

Yan Wu¹, Xiang Qian¹, Shengli Mi¹, Min Zhang¹, Shuqing Sun¹, Xiaohao Wang^1,2

¹Graduate School at Shenzhen, Tsinghua University, ²The State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University

Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.

Environment

Studying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae

Bin Zhang^1,2, Xinyue Yang², Jing Zhao³, Ting Xu^2,4, Daqiang Yin^2,4

¹State Key Laboratory of Marine Geology, Tongji University, ²Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, ³Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Center of 10 Shanghai Polytechnic University, ⁴Shanghai Institute of Pollution Control and Ecological Security

A detailed experimental protocol is presented in this paper for the evaluation of neurobehavioral toxicity of environmental pollutants using a zebrafish larvae model, including the exposure process and tests for neurobehavioral indicators.

Behavior

Individualized rTMS Treatment for Depression using an fMRI-Based Targeting Method

Xin Luo¹, Yiru Hu¹, Runhua Wang¹, Min Zhang¹, Xiaomei Zhong¹, Bin Zhang¹

¹Affiliated Brain Hospital of Guangzhou Medical University

The present protocol describes the application of repetitive transcranial magnetic stimulation (rTMS), where a subregion of the dorsolateral prefrontal cortex (DLPFC) with the strongest functional anticorrelation with the subgenual anterior cingulate cortex (sgACC) was located as the stimulation target under the assistance of a fMRI-based neuronavigation system.

Biology

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Qiang Yan^*1, Yingying He^*2,3, Yang Yue^2,3, Luyao Jie¹, Tingmei Wen^2,3, Yumiao Zhao^2,3, Min Zhang², Tingting Zhang²

¹State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, ²Research Institute of Applied Biology, Shanxi University, ³School of Life Science, Shanxi University

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

Chemistry

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Chunli Song^*1, Zhanfeng Hou^*1,2, Zijun Jiao^*1, Zhaodi Liu³, Chenshan Lian^1,2, Min Zhang¹, Wei Liang³, Feng Yin^1,2, Zigang Li^1,2

¹Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, ²Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, ³Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University

This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.