JoVE Logo

Sign In

Idaho College of Osteopathic Medicine

3 ARTICLES PUBLISHED IN JoVE

image

Biology

Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes
Michelle Z. Chiu 1,2, Steven Lannon 1, Marisol Luchetti 1, Michael B. Wells 3, Deborah J. Andrew 1
1Department of Cell Biology, The Johns Hopkins University School of Medicine, 2Tufts School of Medicine, 3Department of Biomedical Sciences, Idaho College of Osteopathic Medicine

The adult mosquito salivary gland (SG) is required for the transmission of all mosquito-borne pathogens to their human hosts, including viruses and parasites. This video demonstrates efficient isolation of the SGs from the larval (L4) stage Anopheles gambiae mosquitoes and preparation of the L4 SGs for further analysis.

image

Biology

Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes
Mabel Taracena 1,2, Catherine Hunt 1, Pamela Pennington 3, Deborah Andrew 4,5, Marcelo Jacobs-Lorena 5,6, Ellen Dotson 1, Michael Wells 5,7,8
1Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, 2Department of Entomology, Cornell University, 3Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, 4Department of Cell Biology, Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology, Johns Hopkins School of Medicine, 8Biomedical Sciences Department, Idaho College of Osteopathic Medicine

The oral administration of dsRNA produced by bacteria, a delivery method for RNA interference (RNAi) that is routinely used in Caenorhabditis elegans, was successfully applied here to adult mosquitoes. Our method allows for robust reverse genetics studies and transmission-blocking vector studies without the use of injection.

image

Highlighting Molecular Biology as a Focal Point for Mosquito Research
Michael B. Wells 1
1Department of Biomedical Sciences, Idaho College of Osteopathic Medicine

Highlighting Molecular Biology as a Focal Point for Mosquito Research

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved