Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes

3.1K Views

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07:48 min

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March 1st, 2022

DOI :

10.3791/63266-v

March 1st, 2022

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Mabel Taracena¹^,², Catherine Hunt¹, Pamela Pennington³, Deborah Andrew⁴^,⁵, Marcelo Jacobs-Lorena⁵^,⁶, Ellen Dotson¹, Michael Wells⁵^,⁷^,⁸

¹Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, ²Department of Entomology, Cornell University, ³Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, ⁴Department of Cell Biology, Johns Hopkins School of Medicine, ⁵Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, ⁶Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, ⁷Department of Cell Biology, Johns Hopkins School of Medicine, ⁸Biomedical Sciences Department, Idaho College of Osteopathic Medicine

Summary

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Chapters in this video

0:05

Introduction

0:45

Preparation of Heat-Killed Bacteria Expressing Double-Stranded RNA (dsRNA)

2:09

Feeding Mosquitoes with Heat-Killed Bacteria Expressing dsRNA

2:47

Assay Target Gene Expression Levels

3:59

Phenotypic Evaluation: Successful Blood-Feeding

4:35

Phenotypic Evaluation: Salivary Gland Morphology and Down-Regulation of Relevant Proteins

5:30

Results: The Effects of fkh Knockdown in Adult Anopheles gambiae Salivary Glands

7:17

Conclusion

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