A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac AllograftMichelle Mendiola Pla 1, Amy Evans 2, Franklin H. Lee 3, Yuting Chiang 1, Muath Bishawi 1, Andrew Vekstein 1, Lillian Kang 1, Diego Zapata 4, Ryan Gross 3, Alexis Carnes 2, Lynden E. Gault 5, Julie A. Balko 6, Desiree Bonadonna 2, Sam Ho 5, Paul Lezberg 7, Benjamin S. Bryner 1, Jacob N. Schroder 1, Carmelo A. Milano 1, Dawn E. Bowles 3
1Division of Cardiothoracic Surgery, Department of Surgery, Duke University, 2Perfusion Services, Duke University, 3Division of Surgical Sciences, Department of Surgery, Duke University, 4Division of Laboratory Animal Resources, Duke University, 5Gift of Hope Organ and Tissue Donor Network, 6College of Veterinary Medicine, North Carolina State University, 7TransMedics, Inc
We present a protocol for utilizing a normothermic ex vivo sanguinous perfusion system for the delivery of therapeutics to an entire cardiac allograft in a porcine heterotopic heart transplant model.
