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4 ARTICLES PUBLISHED IN JoVE

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Chemistry

A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy
Zhong Wang 1, Benjamin F. Jeffries 1, Hannah R. Felstead 1, Neil J. Wells 1, Elisabetta Chiarparin 2, Bruno Linclau 1
1Chemistry, University of Southampton, 2Medicinal Chemistry, Oncology, IMED Biotech Unit, AstraZeneca

A novel and straightforward variation of the shake-flask method was developed for accurate lipophilicity measurement of fluorinated compounds by 19F NMR spectroscopy.

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Biology

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)
Ida Alexandersson 1, Matthew J. Harms 1, Jeremie Boucher 1,2,3
1Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, 2The Lundberg Laboratory for Diabetes Research, University of Gothenburg, 3Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg

Membrane mature adipocyte aggregate culture (MAAC) is a new method to culture mature human adipocytes. Here we detail how to isolate adipocytes from human adipose and how to set up MAAC.

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Medicine

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Paola Marco-Casanova 1, Natalia Lukashchuk 1, Benedetta Lombardi 1, Veerendra Munugalavadla 2, Melanie M. Frigault 3, Elizabeth A. Harrington 1, J. Carl Barrett 3, Andrew J. Pierce 1
1Translational Medicine, R&D Oncology, AstraZeneca, 2Acerta Pharma, AstraZeneca Group, 3Translational Medicine, R&D Oncology, AstraZeneca

This protocol details clinically implementable preparation of high quality PBMC and plasma biosamples at the clinical trial site that can be used for translational biomarker analysis.

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Genetics

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
Payam Haftbaradaran Esfahani 1, Ralph Knöll 1,2
1Department of Medicine, Integrated Cardio Metabolic Centre (ICMC), Heart and Vascular Theme, Karolinska Institutet, 2Bioscience Cardiovascular, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca

This paper presents methods for growing cardiac myocytes with different shapes, which represent different pathologies, and sorting these adherent cardiac myocytes based on their morphology at a single cell level. The proposed platform provides a novel approach to high throughput and drug screening for different types of heart failure.

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