Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)

18.6K Views

06:28 min

February 13th, 2020

DOI :

10.3791/60485-v

February 13th, 2020


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Human Mature Adipocytes

Chapters in this video

0:05

Introduction

0:53

Human Subcutaneous Adipose Tissue Dissection

1:32

Collagenase Treatment

2:21

Mature Adipocyte Purification

3:20

Mature Adipocyte Packing

3:51

Mature Adipocyte Seeding and Maintenance

4:50

Results: Representative Mature Adipocyte Aggregate Culture (MAAC) Appearance and Response to Diverse Pharmacological Agonism

5:55

Conclusion

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