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Wright State University

15 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Rapid Determination of the Thermal Nociceptive Threshold in Diabetic Rats
Saeed Alshahrani 1, Filipe Fernandez-Conti 2, Amanda Araujo 2, Mauricio DiFulvio 1
1Department of Pharmacology and Toxicology, Wright State University, 2Human Movement Laboratory, Universidade São Judas Tadeu

Here, we describe a rapid reliable and simple procedure to determine the lowest temperature at which rats or mice show nocifensive behavior, i.e. the thermal nociceptive threshold (TNT). This method applies a slowly increasing thermal stimulus allowing precise and reproducible estimation of TNTs with minimum, if any, stress to the animals.

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Biology

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
Catherine B. Anders 1, Joshua D. Baker 1, Adam C. Stahler 1, Austin J. Williams 1, Jackie N. Sisco 2, John C. Trefry 2, Dawn P. Wooley 2, Ioana E. Pavel Sizemore 1
1Department of Chemistry, Wright State University, 2Department of Neuroscience, Cell Biology, and Physiology, Wright State University

Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation.

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JoVE Core

Olfactory Assays for Mouse Models of Neurodegenerative Disease
Andrew M. Lehmkuhl 1, Emily R. Dirr 2,3, Sheila M. Fleming 1,2
1Department of Psychology, University of Cincinnati, 2Department of Neurology, University of Cincinnati, 3Department of Neuroscience, Cell Biology, and Physiology, Wright State University

Impairment in olfactory function is a common feature in many neurodegenerative disorders including Parkinson, Alzheimer, and Huntington diseases. In the present article, we describe a set of tests for assessing olfaction discrimination and detection in mice that can be used to measure olfactory abilities in mouse models of neurodegenerative diseases.

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Immunology and Infection

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
Jennifer N. Gibson 1, Pavani Beesetty 1, Courtney Sulentic 2, J. Ashot Kozak 1
1Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine, Wright State University, 2Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University

T-lymphocyte mitogenesis is accompanied by blastogenic transformation, whereupon the cell volume enlarges before cell division. Here, we describe a method to quantify blastogenesis in T lymphocytes using an automated cell counter with the capability of measuring cell diameters.

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Neuroscience

A Protocol for the Administration of Real-Time fMRI Neurofeedback Training
Matthew S. Sherwood 1,2, Emily E. Diller 2, Elizabeth Ey 3, Subhashini Ganapathy 2,4, Jeremy T. Nelson 5, Jason G. Parker 1,6
1Office of the Vice President for Research and Graduate Studies, Wright State University, 2Department of Biomedical, Industrial and Human Factors Engineering, Wright State University, 3Pediatric Radiology and Medical Imaging, Dayton Children's Hospital, 4Department of Trauma Care and Surgery, Boonshoft School of Medicine, Wright State University, 5Department of Defense Hearing Center of Excellence, JBSA-Lackland, 6Department of Neurology, Boonshoft School of Medicine, Wright State University

The ability to induce and/or control neural plasticity may be critical in future treatments for neurologic disorders and the recovery from brain injury. In this paper, we present a protocol on the use of neurofeedback training with functional magnetic resonance imaging to modulate human brain function.

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Medicine

Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease
Victoria N. Jensen *1, Shannon H. Romer *2, Sarah M. Turner 3, Steven A. Crone 3
1Neuroscience Graduate Program, University of Cincinnati, 2Department of Biological Sciences, Wright State University, 3Division of Neurosurgery, Cincinnati Children's Hospital Medical Center

This paper introduces a method for repeated measurements of ventilation and respiratory muscle activity in a freely behaving amyotrophic lateral sclerosis (ALS) mouse model throughout disease progression with whole-body plethysmography and electromyography via an implanted telemetry device.

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Neuroscience

Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions
Steven R. A. Burke 1, Eric J. Reed 1, Shannon H. Romer 1, Andrew A. Voss 1
1Department of Biological Sciences, Wright State University

The protocol described in this paper uses the mouse levator auris longus (LAL) muscle to record spontaneous and nerve-evoked postsynaptic potentials (current-clamp) and currents (voltage-clamp) at the neuromuscular junction. Use of this technique can provide key insights into mechanisms of synaptic transmission under normal and disease conditions.

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Genetics

Mating-based Overexpression Library Screening in Yeast
Elliott Hayden 1, Shuzhen Chen 1, Abagail Chumley 1, Quan Zhong 1, Shulin Ju 1
1Department of Biological Sciences, Wright State University

This article presents a mating-based method to facilitate overexpression screening in budding yeast using an arrayed plasmid library.

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Biology

Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry
Victoria Wong 1, Prakash Arumugam 1, Lucile E. Wrenshall 1,2
1Department of Neuroscience, Cell Biology, and Physiology, Wright State University, 2Department of Surgery, Boonshoft School of Medicine, Wright State University

Proliferation is a critical part of cellular function, and a common readout used to assess potential toxicity of new drugs. Measuring proliferation is, therefore, a frequently used assay in cell biology. Here we present a simple, versatile method of measuring proliferation that can be used in adherent and non-adherent cells.

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Developmental Biology

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue
Yiding Qi *1, Xiang Miao *2, Lian Xu 3, Mengxia Fu 3, Shi Peng 4, Kailei Shi 5, Jun Li 4, Maoqing Ye 5, Ruogu Li 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University

We present a protocol for the isolation, culture, and adipogenic induction of neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre+/-;Rosa26RFP/+ mice. The NCADSCs can be an easily accessible source of ADSCs for modeling adipogenesis or lipogenesis in vitro.

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Bioengineering

Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy
Chien Poon 1, Ben Rinehart 1, Jun Li 1,2, Ulas Sunar 1
1Department of Biomedical, Industrial and Human Factors Engineering, Wright State University, 2South China Academy of Advanced Optoelectronics, South China Normal University

This protocol demonstrates how to measure resting state functional connectivity in the human prefrontal cortex using a custom-made diffuse correlation spectroscopy instrument. The report also discuss practical aspects of the experiment as well as detailed steps for analyzing the data.

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Medicine

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection
Hang You 1,2, Xin Mao 2, Chenyang Wang 2, Gang Huang 3, Marcus Groettrup 4,5, Jun Li 2
1School of Medicine, Chongqing University, 2Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University, 4Division of Immunology, Department of Biology, University of Konstanz, 5Biotechnology Institute Thurgau, University of Konstanz

The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.

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Medicine

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
Xin Mao *1, Peng Xian *1, Hang You 1,2, Gang Huang 3, Jun Li 1
1Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 2School of Medicine, Chongqing University, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University

In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.

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Medicine

Microscopic Replantation of Penile Glans Amputation Due to Circumcision
Da-Chun Jin *1, Bo Zhou *1, Jun Li 2, Cong-Cong Bao 1, Yong Luo 1, Yong Zhang 1, Peng Wang 1, Gang Bi 1, Yan-Feng Li 1
1Department of Urology, Daping Hospital, Army Medical University, 2Department of Urology, Chongqing 13th People's Hospital

The present protocol describes the emergency management of microscopic replantation of penile glans amputation due to circumcision.

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Bioengineering

Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall
Mieradilijiang Abudupataer *1, Xiujie Yin *1, Bitao Xiang *1, Nan Chen 1, Shiqiang Yan 2, Shichao Zhu 1, Yang Ming 1, Gang Liu 1, Xiaonan Zhou 1, Hao Lai 1, Chunsheng Wang 1, Kai Zhu 1, Jun Li 1
1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 2Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University

Here, we developed a human aorta smooth muscle cell organ-on-a-chip model to replicate the in vivo biomechanical strain of smooth muscle cells in the human aortic wall.

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