University of Belgrade Faculty of Biology

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Katarina Milićević¹, Andrej Korenić¹, Milena Milošević¹, Pavle R. Andjus¹

¹Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”, University of Belgrade Faculty of Biology

We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca²⁺ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1^G93A rat model.