Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral SclerosisKatarina Milićević 1, Andrej Korenić 1, Milena Milošević 1, Pavle R. Andjus 1
1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”, University of Belgrade Faculty of Biology
We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca2+ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1G93A rat model.
