Oklahoma Children's Hospital

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

Guillermo J. Ares¹, Christie Buonpane¹, Carrie Yuan¹, Douglas Wood¹, Catherine J. Hunter^1,2

¹Department of Pediatrics, Northwestern University, ²Division of Pediatric Surgery, Ann & Robert H. Lurie Children's Hospital of Chicago

Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.

Immunology and Infection

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Heather Liebe¹, Camille Schlegel², Xue Cai², Alena Golubkova¹, Tyler Leiva¹, William L. Berry³, Catherine J. Hunter¹

¹Division of Pediatric Surgery, Oklahoma Children's Hospital, ²Oklahoma University Health Sciences Center, ³Department of Surgery, University of Oklahoma Health Sciences Center

The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.