Oklahoma Children's Hospital
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
Guillermo J. Ares 1, Christie Buonpane 1, Carrie Yuan 1, Douglas Wood 1, Catherine J. Hunter 1,2
1Department of Pediatrics, Northwestern University, 2Division of Pediatric Surgery, Ann & Robert H. Lurie Children's Hospital of Chicago
Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.
Immunology and Infection
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
Heather Liebe 1, Camille Schlegel 2, Xue Cai 2, Alena Golubkova 1, Tyler Leiva 1, William L. Berry 3, Catherine J. Hunter 1
1Division of Pediatric Surgery, Oklahoma Children's Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery, University of Oklahoma Health Sciences Center
The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.
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