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INRS-Institut Armand-Frappier

3 ARTICLES PUBLISHED IN JoVE

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Biology

Titration of Human Coronaviruses Using an Immunoperoxidase Assay
Francine Lambert 1, Helene Jacomy 1, Gabriel Marceau 1, Pierre J. Talbot 1
1Laboratory of Neuroimmunovirology, INRS-Institut Armand-Frappier

In this video, we demonstrate an alternative method for detection and titering of viruses using an enzymatic antigen detection technique known as an immunoperoxidase assay. Here, we will show you how to collect your viral samples, prepare the cells for testing, and finally the immunoperoxidase assay using serial dilutions to determine the viral titer.

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Biology

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
Nydia Morales-Soto 1,2, Morgen E. Anyan 1, Anne E. Mattingly 1, Chinedu S. Madukoma 1, Cameron W. Harvey 3, Mark Alber 3, Eric Déziel 4, Daniel B. Kearns 5, Joshua D. Shrout 1,2,6
1Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 2Eck Institute for Global Health, University of Notre Dame, 3Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, 4INRS-Institut Armand-Frappier, 5Department of Biology, Indiana University, 6Department of Biological Sciences, University of Notre Dame

Swarming motility is influenced by physical and environmental factors. We describe a two-phase protocol and guidelines to circumvent the challenges commonly associated with swarm assay preparation and data collection. A macroscopic imaging technique is employed to obtain detailed information on swarm behavior that is not provided by current analysis techniques.

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Developmental Biology

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
Lucas Sagrillo-Fagundes *1, Hélène Clabault *1, Laetitia Laurent *1, Andrée-Anne Hudon-Thibeault 1, Eugênia Maria Assunção Salustiano 1, Marlène Fortier 1, Josianne Bienvenue-Pariseault 1, Philippe Wong Yen 1, J. Thomas Sanderson 1, Cathy Vaillancourt 1
1INRS-Institut Armand-Frappier

This manuscript presents a unique in vitro model of immunopurified human villous cytotrophoblast cells cultured under hypoxia/reoxygenation. This model is suitable to study the protective effects of promising treatments, such as melatonin, on pregnancy complications associated with increased oxidative stress and altered placental function.

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