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Titration of Human Coronaviruses Using an Immunoperoxidase Assay Video (Video) | JoVE

DOI :

10.3791/751-v

12:00 min

April 28th, 2008

April 28th, 2008

37,729 Views

1Laboratory of Neuroimmunovirology, INRS-Institut Armand-Frappier

In this video, we demonstrate an alternative method for detection and titering of viruses using an enzymatic antigen detection technique known as an immunoperoxidase assay. Here, we will show you how to collect your viral samples, prepare the cells for testing, and finally the immunoperoxidase assay using serial dilutions to determine the viral titer.

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Titration

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