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Saint Louis University School of Medicine

7 ARTICLES PUBLISHED IN JoVE

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Biology

Fabrication of Amperometric Electrodes
Carolyn M. Pike 1, Chad P. Grabner 2, Amy B. Harkins 1
1Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, 2Yale University School of Medicine

This protocol describes how to generate carbon fiber electrodes. The electrodes are subsequently used to detect catecholamine release from vesicles with carbon fiber amperometry.

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Biology

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
James D. Shoemaker 1
1Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine

The urease method of sample preparation for GC/MS analysis of intermediary metabolites is presented by its inventor. The method allows one-step follow-up of newborn screening for inborn errors by tandem mass spectrometry by quantifying carbohydrates, organic and amino acids all in a single process.

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Biology

Isolation and Primary Culture of Rat Hepatic Cells
Ling Shen 1, Allix Hillebrand 2, David Q.-H. Wang 3, Min Liu 1
1Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 2American University in Washington, D.C., 3Department of Internal Medicine, Saint Louis University School of Medicine

Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.

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Medicine

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
Will Sewell 1, Ashley Reeb 1, Reigh-Yi Lin 1
1Department of Internal Medicine, Division of Endocrinology, Saint Louis University School of Medicine

Generation of an orthotopic mouse model of anaplastic thyroid carcinoma is described here. This technique employs surgical placement of human anaplastic thyroid cancer cells into the thyroid of immunodeficient mice, thus creating a more clinically relevant setting to study disease progression as well as screen innovative therapeutic interventions.

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Neuroscience

Functional Analysis of the Larval Feeding Circuit in Drosophila
Parag K. Bhatt 1, Wendi S. Neckameyer 1
1Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine

The feeding circuit in Drosophila melanogaster larvae serves a simple yet powerful model that allows changes in feeding rate to be correlated with alterations in the stomatogastric neural circuitry. This circuit is composed of central serotonergic neurons that send projections to the mouth hooks as well as the foregut.

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Labeling Neuronal Morphology Using Custom Diolistic Techniques
Lyon Hough 1, Michael E. Brown 2
1Department of Biomedical Sciences, Missouri State University, 2Department of Pathology, Saint Louis University School of Medicine

Here, we present a protocol for custom diolistic labeling. The customization of this fluorescent neuronal labeling method provides a modifiable technique that can be adapted to a wide variety of research goals and applications in the analysis of neuronal morphology.

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Immunology and Infection

Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
Nermi L. Parrow *1, Pierre-Christian Violet *2, Hongbin Tu 2, James Nichols 3, Corinne A. Pittman 4, Courtney Fitzhugh 4, Robert E. Fleming 1,5, Narla Mohandas 6, John F. Tisdale 3, Mark Levine 2
1Department of Pediatrics, Saint Louis University School of Medicine, 2Molecular and Clinical Nutrition Section, Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, 3Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, 4Sickle Cell Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 5Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 6Red Cell Physiology Laboratory, New York Blood Center

Here we present techniques to measure red cell deformability and cellular heterogeneity by ektacytometry. These techniques are applicable to general investigations of red cell deformability and specific investigations of blood diseases characterized by the presence of both rigid and deformable red cells in circulation, such as sickle cell anemia.

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