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University of Medicine and Dentistry of New Jersey

5 ARTICLES PUBLISHED IN JoVE

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Biology

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
Nadine H. Soplop 1,2, Rajesh Patel 1, Sunita G. Kramer 1,2
1Joint Graduate Program in Cell and Developmental Biology, UMDNJ-Graduate School of Biomedical Sciences and Rutgers: The State University of New Jersey, 2Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey

We describe a process for fixation, embedding, sectioning, and imaging of late stage Drosophila embryos for Trasmission Electron Microscopy of the embryonic heart tube. This technique allows for the visualization of the heart tube lumen as well as the basement membrane, which lines the lumen of the heart.

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Bioengineering

Axon Stretch Growth: The Mechanotransduction of Neuronal Growth
Joseph R. Loverde 1,2, Rosa E. Tolentino 1,2, Bryan J. Pfister 1
1Departments of Biomedical Engineering, New Jersey Institute of Technology, 2Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey

A unique tissue engineering method was developed to elongate numerous nerve fibers in culture by recapitulating axon stretch growth; a form of nervous system growth whereby nerves elongate in conjunction with growth of the enlarging body.

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Medicine

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
Chung Wong 1, Evan Vosburgh 1,2, Arnold J. Levine 2,3, Lei Cong 2, Eugenia Y. Xu 1,2
1Raymond and Beverly Sackler Foundation, 2The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, 3School of Natural Sciences, Institute for Advanced Study, Princeton, New Jersey

We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.

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Neuroscience

A Caenorhabditis elegans Model System for Amylopathy Study
Zhibing Duan 1, Federico Sesti 1
1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey

We describe methods to study aspects of amylopathies in the worm C. elegans. We show how to construct worms expressing human Aβ42 in neurons and how to test their function in behavioral assays. We further show how to obtain primary neuronal cultures that can be used for pharmacological testing.

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Biology

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately
Wenjin Chen 2,3, Chung Wong 1,3, Evan Vosburgh 1,3, Arnold J. Levine 3,4, David J. Foran 2,3, Eugenia Y. Xu 1,3
1Raymond and Beverly Sackler Foundation, New Jersey, 2Histopathology and Imaging Shared Resource, Rutgers University, 3Rutgers Cancer Institute of New Jersey, Rutgers University, 4School of Natural Sciences, Institute for Advanced Study, New Jersey

We present a high-throughput image analysis software application to measure the size of three-dimensional tumor spheroids imaged with bright-field microscopy. This application provides a fast and effective way to examine the effects of therapeutic drugs on spheroids, which is beneficial for researchers who wish to use spheroids in drug screens.

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