Salk Institute for Biological Studies

3 ARTICLES PUBLISHED IN JoVE

Biology

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Bartosz Balana¹, Natalie Taylor¹, Paul A. Slesinger¹

¹Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies

We will demonstrate how to study the effect of a single point mutation on the function of an ion channel.

Neuroscience

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection

Tatiana Hurtado de Mendoza¹, Bartosz Balana², Paul A. Slesinger², Inder M. Verma¹

¹Laboratory of Genetics, The Salk Institute for Biological Studies, ²Clayton Foundation Laboratories for Peptide Biology, The Salk Institute for Biological Studies

This method describes the generation of organotypic cerebellar cultures and the effect of certain apoptotic stimuli on the viability of different cerebellar cell types.

Neuroscience

Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo

Emre Lacin¹, Arnaud Muller², Marian Fernando¹, David Kleinfeld^2,3, Paul A. Slesinger¹

¹Department of Neuroscience, Icahn School of Medicine at Mount Sinai, ²Department of Physics, University of California, San Diego, ³Section of Neurobiology, University of California, San Diego

We present a protocol to create cell-based neurotransmitter fluorescent engineered reporters (CNiFERs) for the optical detection of volumetric neurotransmitter release.