Salk Institute for Biological Studies
3 ARTICLES PUBLISHED IN JoVE
Biology
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Bartosz Balana 1, Natalie Taylor 1, Paul A. Slesinger 1
1Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies
We will demonstrate how to study the effect of a single point mutation on the function of an ion channel.
Neuroscience
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
Tatiana Hurtado de Mendoza 1, Bartosz Balana 2, Paul A. Slesinger 2, Inder M. Verma 1
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Clayton Foundation Laboratories for Peptide Biology, The Salk Institute for Biological Studies
This method describes the generation of organotypic cerebellar cultures and the effect of certain apoptotic stimuli on the viability of different cerebellar cell types.
Neuroscience
Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo
Emre Lacin 1, Arnaud Muller 2, Marian Fernando 1, David Kleinfeld 2,3, Paul A. Slesinger 1
1Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 2Department of Physics, University of California, San Diego, 3Section of Neurobiology, University of California, San Diego
We present a protocol to create cell-based neurotransmitter fluorescent engineered reporters (CNiFERs) for the optical detection of volumetric neurotransmitter release.
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