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Ulm University

13 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
Wolfgang Stein 1, Carola Städele 1, Peter Andras 2
1Institute of Neurobiology, Ulm University, 2School of Computing Science & Institute of Neuroscience, Newcastle University

Here we present the methodology for fast and high resolution fluorescent voltage-sensitive dye imaging of detailed activity of neurons in the crab stomatogastric ganglion.

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Medicine

Diffusion Tensor Magnetic Resonance Imaging in the Analysis of Neurodegenerative Diseases
Hans-Peter Müller 1, Jan Kassubek 1
1Department of Neurology, University of Ulm

Diffusion tensor imaging (DTI) basically serves as an MRI-based tool to identify in vivo the microstructure of the brain and pathological processes due to neurological disorders within the cerebral white matter. DTI-based analyses allow for application to brain diseases both at the group level and in single subject data.

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Medicine

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Touraj Shokati 1, Nicholas Bodenberger 1, Holly Gadpaille 1, Björn Schniedewind 1, Alexander A. Vinks 2, Wenlei Jiang 3, Rita R. Alloway 4, Uwe Christians 1
1iC42 Clinical Research and Development, University of Colorado, Anschutz Medical Campus, 2Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, 3Food and Drug Administration (FDA), Center of Drug Evaluation Research - Office of Generic Drugs, 4Transplant Clinical Research, University of Cincinnati

Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.

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Developmental Biology

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
Sathish Venkataramanappa 1, Ruth Simon 1, Stefan Britsch 1
1Institute of Molecular and Cellular Anatomy, University of Ulm

Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.

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Developmental Biology

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
Daniel Wehner *1, Christopher Jahn *1, Gilbert Weidinger 1
1Institute for Biochemistry and Molecular Biology, Ulm University

Here we outline the workflow for using the TetON system to achieve tissue-specific gene expression in the adult regenerating zebrafish tail fin.

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Behavior

Eye-Tracking Control to Assess Cognitive Functions in Patients with Amyotrophic Lateral Sclerosis
Jürgen Keller 1, Martin Gorges 1, Helena E. A. Aho-Özhan 1, Ingo Uttner 1, Erich Schneider 2, Jan Kassubek 1, Elmar H. Pinkhardt 1, Albert C. Ludolph 1, Dorothée Lulé 1
1Department of Neurology, Ulm University, 2Institute of Medical Technology, Brandenburg University of Technology Cottbus-Senftenberg

Cognitive deficits are common in about one third of patients with amyotrophic lateral sclerosis, a neurological condition leading to progressive impairments in speech and movement abilities. To conduct cognitive tests in patients unable to speak or write a reliable and easy to administer eye-tracking paradigm was developed.

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Biochemistry

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
Thilo Dörfler *1, Tobias Eilert *1, Carlheinz Röcker 1, Julia Nagy 1, Jens Michaelis 1
1Institute of Biophysics, Ulm University

We present the setup and experimental procedure to obtain smFRET data from large donor-acceptor networks with a TIRF microscope. The step-by-step analysis of these measurements with the Bayesian inference software Fast-NPS yields high-resolved structural information via the application of adapted dye models.

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Biochemistry

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
Nicholas Bodenberger 1, Dennis Kubiczek 1, Frank Rosenau 1
1Center for Peptide Pharmaceuticals, Faculty of Natural Science, Ulm University

Different methods to manipulate three-dimensional architecture in protein-based hydrogels are evaluated here with respect to material properties. The macroporous networks are functionalized with a cell-adhesive peptide, and their feasibility in cell culture is evaluated using two different model cell lines.

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Neuroscience

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
Christoph Wiegreffe 1, Svenja Feldmann 1, Simeon Gaessler 1, Stefan Britsch 1
1Institute of Molecular and Cellular Anatomy, Ulm University

This protocol provides instructions for direct observation of radially migrating cortical neurons. In utero electroporation, organotypic slice culture, and time-lapse confocal imaging are combined to directly and dynamically study the effects of overexpression or downregulation of genes of interest in migrating neurons and to analyze their differentiation during development.

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Behavior

Use of a Psychophysiological Script-driven Imagery Experiment to Study Trauma-related Dissociation in Borderline Personality Disorder
Dana Maria Bichescu-Burian 1, Benjamin Grieb 1, Tilman Steinert 1, Carmen Uhlmann 1, Jürgen Steyer 1
1Centre for Psychiatry Weissenau, Clinic of Psychiatry and Psychotherapy I, Ulm University

We present a protocol of personalized script-driven trauma-related imagery and clinical assessments within a comparison design for investigating peritraumatic dissociation (PD), psychophysiological reactions, i.e. heart rate (HR) and skin conductance (SC), and psychological features of often severely traumatized individuals with borderline personality disorder (BPD).

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Bioengineering

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
Rachel Santarella-Mellwig 1, Uta Haselmann 2, Nicole L. Schieber 1, Paul Walther 3, Yannick Schwab 1, Claude Antony 1, Ralf Bartenschlager 2,4, Inés Romero-Brey 2
1European Molecular Biology Laboratory, 2Department of Infectious Diseases, Molecular Virology, Heidelberg University, 3Central Facility for Electron Microscopy, Ulm University, 4Heidelberg Partner Site, German Center for Infection Research

A correlative light electron microscopy (CLEM) method is applied to image virus-induced intracellular structures via electron microscopy (EM) in cells that are previously selected by light microscopy (LM). LM and EM are combined as a hybrid imaging approach to achieve an integrated view of virus-host interactions.

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Behavior

Multi-Modal Signals for Analyzing Pain Responses to Thermal and Electrical Stimuli
Sascha Gruss 1, Mattis Geiger 2, Philipp Werner 3, Oliver Wilhelm 2, Harald C. Traue 1, Ayoub Al-Hamadi 3, Steffen Walter 1
1Department of Medical Psychology, Ulm University, 2Department of Individual Differences and Psychological Assessment, Ulm University, 3Department of Neuro-Information Technology, University of Magdeburg

This article focuses on the experimental elicitation of pain through heat (thermal) and electrical stimulation while recording physiological, visual, and paralinguistic responses. It aims at collecting valid multimodal data for analyzing pain based on its intensity, quality, and duration. 

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Engineering

Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios
Burkhard Hoppenstedt 1, Thomas Probst 2, Manfred Reichert 1, Winfried Schlee 3, Klaus Kammerer 1, Myra Spiliopoulou 4, Johannes Schobel 1, Michael Winter 1, Anna Felnhofer 5, Oswald D. Kothgassner 6, Rüdiger Pryss 7
1Institute of Databases and Information Systems, Ulm University, 2Department for Psychotherapy and Biopsychosocial Health, Danube University Krems, 3Department of Psychiatry and Psychotherapy, University of Regensburg, 4Faculty of Computer Science, Otto von Guericke University Magdeburg, 5Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 6Department of Child and Adolescent Psychiatry, Medical University of Vienna, 7Institute of Clinical Epidemiology and Biometry, University of Würzburg

This protocol delineates the technical setting of a developed mixed reality application that is used for immersive analytics. Based on this, measures are presented, which were used in a study to gain insights into usability aspects of the developed technical solution.

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