Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

12.0K Views

12:30 min

February 9th, 2017

DOI :

10.3791/54782-v

February 9th, 2017


Transcript

Explore More Videos

Single molecule FRET

Chapters in this video

0:05

Title

0:57

Single-molecule FRET (smFRET) Measurements with Total Internal Reflection Fluorescence (TIRF) Microscopy

5:19

Determination of Mean FRET Efficiency

6:45

Determination of Credible Volumes with the Fast Nano-positioning System (Fast-NPS)

10:09

Results: smFRET Fast Nano-positioning System Modeling of Archaeal RNA Polymerase Open Promotor Complex

11:42

Conclusion

Related Videos

article

11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

10.6K Views

article

11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

10.0K Views

article

10:05

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

3.9K Views

article

13:01

gP2S, an Information Management System for CryoEM Experiments

5.3K Views

article

07:12

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

3.3K Views

article

08:57

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

7.1K Views

article

10:59

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

3.1K Views

article

01:49

Author Spotlight: Unlocking the World of Intrinsically Disordered Regions with Cellular Sensing and Responses

700 Views

article

02:07

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

800 Views

article

01:17

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

911 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved