The City College of New York

10 ARTICLES PUBLISHED IN JoVE

Neuroscience

Electrode Positioning and Montage in Transcranial Direct Current Stimulation

Alexandre F. DaSilva¹, Magdalena Sarah Volz^2,3, Marom Bikson⁴, Felipe Fregni²

¹Headache & Orofacial Pain Effort (H.O.P.E.), Biologic & Material Sciences, School of Dentistry, University of Michigan , ²Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, ³Charité, University Medicine Berlin, ⁴Department of Biomedical Engineering, The City College of New York

Transcranial direct current stimulation (tDCS) is an established technique to modulate cortical excitability^1,2. It has been used as an investigative tool in neuroscience due to its effects on cortical plasticity, easy operation, and safe profile. One area that tDCS has been showing encouraging results is pain alleviation ^3-5.

Medicine

Technique and Considerations in the Use of 4x1 Ring High-definition Transcranial Direct Current Stimulation (HD-tDCS)

Mauricio F. Villamar^1,2, Magdalena Sarah Volz^1,3, Marom Bikson⁴, Abhishek Datta^1,4, Alexandre F. DaSilva^*5, Felipe Fregni^*1

¹Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, ²School of Medicine, Pontifical Catholic University of Ecuador, ³Charité University Medicine Berlin, ⁴The City College of The City University of New York, ⁵Headache & Orofacial Pain Effort (H.O.P.E.), Biologic & Materials Sciences, School of Dentistry, University of Michigan

High-definition transcranial direct current stimulation (HD-tDCS), with its 4x1-ring montage, is a noninvasive brain stimulation technique that combines both the neuromodulatory effects of conventional tDCS with increased focality. This article provides a systematic demonstration of the use of 4x1 HD-tDCS, and the considerations needed for safe and effective stimulation.

Bioengineering

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Michelle T. Juarez¹, Rachel A. Patterson², Wilson Li², William McGinnis²

¹Sophie Davis School of Biomedical Education, The City College of New York, ²Cell & Developmental Biology, University of California, San Diego

The embryonic epidermis of very late stage Drosophila embryos provides an in vivo system for rapid puncture wound response analysis and can be combined with genetic manipulations or chemical microinjection treatments to advance studies in wound healing for translation into mammalian models.

Medicine

A Protocol for the Use of Remotely-Supervised Transcranial Direct Current Stimulation (tDCS) in Multiple Sclerosis (MS)

Margaret Kasschau^1,2, Kathleen Sherman^1,2, Lamia Haider², Ariana Frontario^1,2, Michael Shaw^1,2, Abhishek Datta³, Marom Bikson⁴, Leigh Charvet^1,2

¹Multiple Sclerosis Comprehensive Care Center, Department of Neurology, NYU Langone Medical Center, ²Department of Neurology, Stony Brook Medicine, ³Soterix Medical, Inc, ⁴Department of Biomedical Engineering, The City College of New York

The goal of this pilot study is to describe a protocol for the remotely-supervised delivery of transcranial direct current stimulation (tDCS) so that the procedure maintains standards of in-clinic practice, including safety, reproducibility, and tolerability. The feasibility of this protocol was tested in participants with multiple sclerosis (MS).

Neuroscience

Remotely Supervised Transcranial Direct Current Stimulation: An Update on Safety and Tolerability

Michael T. Shaw¹, Margaret Kasschau², Bryan Dobbs¹, Natalie Pawlak¹, William Pau¹, Kathleen Sherman¹, Marom Bikson³, Abhishek Datta⁴, Leigh E. Charvet¹

¹New York University, Langone Medical Center, ²Stony Brook Medicine, ³City College of New York, ⁴Soterix Medical

This manuscript provides an updated remote supervision protocol that enables participation in transcranial direct current stimulation (tDCS) clinical trials while receiving treatment sessions from home. The protocol has been successfully piloted in both patients with multiple sclerosis and Parkinson's disease.

Neuroscience

Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain

Reem Khalil¹, Jonathan B. Levitt²

¹Department of Biology, Chemistry and Environmental Sciences, American University of Sharjah, ²Department of Biology, The City College of New York

We describe a histochemical procedure that reveals characteristic laminar and areal zinc staining patterns in different brain regions. The zinc-staining pattern may be used in conjunction with other anatomical markers to reliably distinguish layers and regions in the developing and adult brain.

Neuroscience

Laboratory Administration of Transcutaneous Auricular Vagus Nerve Stimulation (taVNS): Technique, Targeting, and Considerations

Bashar W. Badran^*1,2,3, Alfred B. Yu^*2, Devin Adair¹, Georgia Mappin³, William H. DeVries³, Dorothea D. Jenkins⁴, Mark S. George^3,5,6, Marom Bikson¹

¹Department of Biomedical Engineering, City College of New York, ²U.S. Army Research Laboratory, Aberdeen Proving Ground, ³Brain Stimulation Laboratory, Department of Psychiatry, Medical University of South Carolina, ⁴Department of Pediatrics, Medical University of South Carolina, ⁵Department of Neurology, Medical University of South Carolina, ⁶Ralph H. Johnson VA Medical Center

A methodological description of the technique, potential targets, and proper administration of transcutaneous auricular vagus nerve stimulation (taVNS) on the human ear is described.

Medicine

Updated Technique for Reliable, Easy, and Tolerated Transcranial Electrical Stimulation Including Transcranial Direct Current Stimulation

Helen Borges¹, Alexandra Dufau^1,2, Bhaskar Paneri¹, Adam J. Woods³, Helena Knotkova^4,5, Marom Bikson¹

¹Department of Biomedical Engineering, The City College of New York, CUNY, ²Department of Clinical and Health Psychology, Center for Cognitive Aging and Memory, ³McKnight Brain Institute, University of Florida, ⁴MJHS Institute for Innovation in Palliative Care, ⁵Department of Family and Social Medicine, Albert Einstein College of Medicine

When administering transcranial direct current stimulation (tDCS), reproducible electrode preparation and placement are vital for a tolerated and effective session. The purpose of this article is to demonstrate updated modern setup procedures for the administration of tDCS and related transcranial electrical stimulation techniques, such as transcranial alternating current stimulation (tACS).

Behavior

Transcranial Direct Current Stimulation for Online Gamers

Sang Hoon Lee^*1, Jooyeon Jamie Im^*2, Jin Kyoung Oh², Eun Kyoung Choi², Sujung Yoon³, Marom Bikson⁴, In-Uk Song⁵, Hyeonseok Jeong², Yong-An Chung²

¹Department of Radiology, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ²Department of Radiology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ³Department of Brain and Cognitive Sciences, Ewha Womans University, ⁴Department of Biomedical Engineering, The City College of New York, ⁵Department of Neurology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea

We present a protocol and a feasibility study for applying transcranial direct current stimulation (tDCS) and neuroimaging assessment in online gamers.

Bioengineering

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

Karl J. Lewis¹, James F. Boorman-Padgett³, Macy Castaneda², David C. Spray⁴, Mia M. Thi^5,6, Mitchell B. Schaffler³

¹Meinig School of Biomedical Engineering, Cornell University, ²Sibley School of Mechanical and Aerospace Engineering, Cornell University, ³Department of Biomedical Engineering, The City College of New York, ⁴Department of Neuroscience, Albert Einstein College of Medicine, ⁵Department of Orthopaedic Surgery, Albert Einstein College of Medicine, ⁶Department of Molecular Pharmacology, Albert Einstein College of Medicine

The current article describes performing an intravital imaging approach to observe mechanically induced calcium signaling of embedded osteocytes in vivo in real-time in response to tissue-level mechanical loading of the mouse third metatarsal.