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LaJolla Institute for Allergy and Immunology

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Flow Cytometry Analysis of Immune Cells Within Murine Aortas
Matthew J. Butcher 1, Margo Herre 1, Klaus Ley 2, Elena Galkina 1
1Deptartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 2Division of Inflammation Biology, LaJolla Institute for Allergy and Immunology

This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.

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