JoVE Logo
Faculty Resource Center

Sign In

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

DOI :

10.3791/2848-v

July 1st, 2011

July 1st, 2011

33,936 Views

1Deptartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 2Division of Inflammation Biology, LaJolla Institute for Allergy and Immunology

This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.

Tags

Flow Cytometry

-- Views

Related Videos

article

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

article

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

article

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

article

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

article

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

article

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

article

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

article

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

article

Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis

article

Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved