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Mary Babb Randolph Cancer Center, West Virginia University

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
Amanda Gatesman Ammer 1, Karen E. Hayes 1, Karen H. Martin 1, Lingqing Zhang 2, George A. Spirou 3, Scott A. Weed 1
1Department of Neurobiology and Anatomy, Program in Cancer Cell Biology, Mary Babb Randolph Cancer Center, West Virginia University, 2Sensory Neuroscience Research Center, West Virginia University , 3Departments of Otolaryngology and Physiology, Center for Neuroscience, West Virginia University

A comprehensive overview of the techniques involved in generating a mouse model of oral cancer and quantitative monitoring of tumor invasion within the tongue through multi-photon microscopy of labeled cells is presented. This system can serve as a useful platform for the molecular assessment and drug efficacy of anti-invasive compounds.

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Biology

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
Karen H. Martin 1, Karen E. Hayes 1, Elyse L. Walk 1, Amanda Gatesman Ammer 1, Steven M. Markwell 1, Scott A. Weed 1
1Department of Neurobiology and Anatomy, Program in Cancer Cell Biology, Mary Babb Randolph Cancer Center, West Virginia University

We describe the prototypical method for producing microscope coverslips coated with fluorescent gelatin for visualizing invadopodia-mediated matrix degradation. Computational techniques using available software are presented for quantifying the resultant levels of matrix proteolysis by single cells within a mixed population and for multicellular groups encompassing entire microscopic fields.

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Medicine

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
Elyse L. Walk *1,3, Sarah L. McLaughlin *2,3, Scott A. Weed 1,3
1Department of Neurobiology and Anatomy, West Virginia University, 2Animal Models and Imaging Facility, West Virginia University, 3Mary Babb Randolph Cancer Center, West Virginia University

This protocol describes the application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph nodes. This technique optimizes visualization and quantification of cervical lymph node morphology, volume and blood flow. Image-guided biopsy of cervical lymph nodes and processing of lymph tissue for histological evaluation is also demonstrated.

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Medicine

Modeling Chemotherapy Resistant Leukemia In Vitro
William L. Slone *1, Blake S. Moses *1, Rebecca Evans 1, Debbie Piktel 1, Karen H. Martin 1,2, William Petros 1, Michael Craig 1, Laura F. Gibson 1,3
1Alexander B. Osborn Hematopoietic Malignancy and Transplantation Program of the Mary Babb Randolph Cancer Center, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, 2Department of Neurobiology and Anatomy, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, 3Department of Microbiology, Immunology and Cell Biology, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine

The current report summarizes a protocol that can be utilized to model the influence of the bone marrow microenvironment niche on leukemic cells with emphasis placed on enrichment of the most chemoresistant subpopulation.

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