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University of Texas Health Science Center at Houston

7 ARTICLES PUBLISHED IN JoVE

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Medicine

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology
Madonna M. McManus 1, Raymond J. Grill 1
1Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston

A longitudinal examination of bone loss in the femurs and tibiae of adult mice was performed following spinal cord injury using sequential low-dose X-ray scans. Tibia bone loss was detected throughout the study, while bone loss in the femur was not detected until 40 days post injury.

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Medicine

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Hong Jiang 1, Deepa Parthasarathy 1, Ashley C. Torregrossa 1, Asad Mian 2, Nathan S. Bryan 1
1Texas Therapeutics Institute, University of Texas Health Science Center at Houston , 2Deptartment of Pediatrics, Baylor College of Medicine

The endogenous production of nitric oxide (NO) regulates a wide variety of biological functions. It is becoming increasingly clear that disruption or dysregulation of NO based signaling is involved in many human diseases. Methods to quantify relevant NO metabolites may provide novel diagnostic or prognostic biomarkers for human disease.

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Medicine

Breathing-controlled Electrical Stimulation (BreEStim) for Management of Neuropathic Pain and Spasticity
Sheng Li 1,2,3
1Department of Physical Medicine and Rehabilitation, University of Texas Health Science Center at Houston , 2UTHealth Motor Recovery Laboratory, TIRR Memorial Hermann Hospital, 3The Institute of Rehabilitation and Research (TIRR), TIRR Memorial Hermann Hospital

The purpose is to present a new method, breathing-control electrical stimulation (BreEStim) for management of neuropathic pain and spasticity.

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Medicine

Working with Human Tissues for Translational Cancer Research
Alexandre Reuben *1, Vancheswaran Gopalakrishnan *1, Heidi E. Wagner 3, Christine N. Spencer 2, Jacob Austin-Breneman 1, Hong Jiang 1, Zachary A. Cooper 1, Jennifer A. Wargo 1
1Department of Surgical Oncology, University of Texas MD Anderson Cancer Center, 2Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, 3Department of Pathology and Institutional Tissue Bank, University of Texas MD Anderson Cancer Center

Translational cancer research is dependent on extraction of human tissues. Much work has gone into optimizing extraction methods for ex vivo analysis. Here, we describe tissue processing methods allowing for maximal data output from limited samples.

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Chemistry

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
Sura Kim 1, Jayoung Ku 1,2, Jaemin Park 1, Raisa Kharbash 1,2, Sheng Li 1
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 2KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

A protocol for the preparation of poly(pentafluorophenyl acrylate) (poly(PFPA)) grafted silica beads is presented. The poly(PFPA) functionalized surface is then immobilized with antibodies and used successfully for the protein separation through immunoprecipitation.

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Developmental Biology

Applications of RNA Interference in American Cockroach
Liang Li *1, Andi Jing *1, Minxin Xie 1, Sheng Li 1,2, Chonghua Ren 1,2
1Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, 2Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University

The present protocol describes step-by-step guidelines for the RNAi operation techniques in P. americana.

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Medicine

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Hong Jiang *1, Yating Zhang *1, Ya Hou 1, Lijun Li 2, Sanyin Zhang 3,4, Yi Zhang 5, Xianli Meng 1,3,4, Xiaobo Wang 4
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine

The present protocol describes an efficient method for the real-time and dynamic acquisition of voltage-gated potassium (Kv) channel currents in H9c2 cardiomyocytes using the whole-cell patch-clamp technique.

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