Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
2.7K Views
•
08:11 min
•
November 11th, 2022
DOI :
November 11th, 2022
•Hong Jiang*1, Yating Zhang*1, Ya Hou1, Lijun Li2, Sanyin Zhang3,4, Yi Zhang5, Xianli Meng1,3,4, Xiaobo Wang4
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine
Explore More Videos
Chapters in this video
0:04
Introduction
1:04
Cell Culture
1:41
Fabrication of Micropipettes
2:16
Instrument Setup
2:37
IKv Parameter Setting
3:16
Whole‐Cell Patch‐Clamp Recording of the IKv in Voltage‐Clamp Mode
6:43
Results: IKv in H9c2 Cells
7:37
Conclusion
Related Videos
14:39
Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
17.2K Views
10:08
Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
2.0K Views
10:19
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
20.9K Views
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
32.3K Views
11:02
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
15.1K Views
11:33
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
13.3K Views
10:53
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
20.3K Views
12:52
Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
7.9K Views
10:30
Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
2.7K Views
06:22
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
3.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved