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Erasmus Medical Center

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Wendy L.J. Hansen 1, Judith Beuving 1, Annelies Verbon 2, Petra. F.G. Wolffs 1
1Department of Medical Microbiology, Maastricht University Medical Center, 2Department of Internal Medicine, Erasmus Medical Center

The design of a straightforward one-day workflow scheme for bacterial pathogen diagnostics enables the rapid recognition of bloodstream infections. The inclusion of eight clinically relevant bacterial targets and their antibiotic resistance profiles offers the clinician an initial insight on the same day, which can lead to more adequate therapy.

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Medicine

Optical Clearing and Imaging of Immunolabeled Kidney Tissue
Turgay Saritas 1, Victor G. Puelles 1,2,3, Xiao-Tong Su 4, David H. Ellison 4,5,6, Rafael Kramann 1,7
1Division of Nephrology and Clinical Immunology, University Hospital RWTH Aachen, 2III. Department of Medicine, University Medical Center, Hamburg-Eppendorf, 3Department of Nephrology, Monash Health, 4Division of Nephrology and Hypertension, Oregon Health and Science University, 5Renal Section, Veterans Affairs Portland Health Care System, 6Fondation LeDucq Transatlantic Networks of Excellence, 7Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical Center

The combination of antibody labeling, optical clearing, and advanced light microscopy allows three-dimensional analysis of complete structures or organs. Described here is a simple method to combine immunolabeling of thick kidney slices, optical clearing with ethyl cinnamate, and confocal imaging that enables visualization and quantification of three-dimensional kidney structures.

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Genetics

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR
Antonio Mancarella 1, Francesco A. Procopio 1, Tilmann Achsel 2, Elisa De Crignis 3, Brian T. Foley 4, Giampietro Corradin 5, Claudia Bagni 2, Giuseppe Pantaleo 1, Cecilia Graziosi 1
1Division of Immunology and Allergy, Lausanne University Hospital, 2Department of Fundamental Neuroscience, University of Lausanne, 3Department of Biochemistry, Erasmus Medical Center, 4Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, 5Department of Biochemistry, University of Lausanne

RNA hairpins and loops can function as primers for reverse transcription (RT) in absence of sequence-specific primers, interfering with the study of overlapping antisense transcripts. We have developed a technique able to identify strand-specific RNA, and we have used it to study HIV-1 antisense protein ASP.

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Bioengineering

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue
Hanneke Crielaard 1, Su Guvenir Torun 1, Tamar B. Wissing 1,2, Pablo de Miguel Muñoz 1,3, Gert-Jan Kremers 4, Frank J. H. Gijsen 1,3, Kim Van Der Heiden 1,2, Ali C. Akyildiz 1,3
1Department of Biomedical Engineering, Erasmus Medical Center, 2Department of Biomedical Engineering, Eindhoven University of Technology, 3Department of Biomechanical Engineering, Delft University of Technology, 4Erasmus Optical Imaging Center, Erasmus Medical Center

We have developed a mechano-imaging pipeline to study the heterogeneous structural and mechanical atherosclerotic plaque properties. This pipeline enables correlation of the local predominant angle and dispersion of collagen fiber orientation, the rupture behavior, and the strain fingerprints of the fibrous plaque tissue.

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Developmental Biology

Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing
Naomi J. M. Kakiailatu 1, Laura E. Kuil 1,2, Eric Bindels 3, Joke T. M. Zink 3, Michael Vermeulen 3, Veerle Melotte 4, Maria M. Alves 1
1Department of Clinical Genetics, Erasmus University Medical Center - Sophia Children's Hospital, 2Division of Psychosocial Research and Epidemiology, the Netherlands Cancer Institute, 3Department of Hematology, Erasmus Medical Center, 4Department of Pathology, GROW-School for Oncology and Developmental Biology, Maastricht University Medical Center

Here, we describe a method for gut isolation from zebrafish larvae at 5 days post fertilization, for single-cell RNA sequencing analysis.

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