Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR
7.4K Views
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08:01 min
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November 27th, 2019
DOI :
November 27th, 2019
•Antonio Mancarella1, Francesco A. Procopio1, Tilmann Achsel2, Elisa De Crignis3, Brian T. Foley4, Giampietro Corradin5, Claudia Bagni2, Giuseppe Pantaleo1, Cecilia Graziosi1
1Division of Immunology and Allergy, Lausanne University Hospital, 2Department of Fundamental Neuroscience, University of Lausanne, 3Department of Biochemistry, Erasmus Medical Center, 4Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, 5Department of Biochemistry, University of Lausanne
Chapters in this video
0:04
Title
1:14
Reverse Transcription and RNA Isolation
2:49
Affinity Purification of ASP Biotinylated cDNA
4:29
Standard PCR
5:10
Results: ASP RNA Detection in CD4+ Cells from HIV Patients
6:56
Conclusion
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