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University of Rochester

2 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium
Carleen M. Bowers 1, Eric J. Toone 1, Robert L. Clark 2, Alexander A. Shestopalov 3
1Department of Chemistry, Duke University , 2Hajim School of Engineering and Applied Sciences, University of Rochester , 3Department of Chemical Engineering, University of Rochester

Here we describe a simple method for patterning oxide-free silicon and germanium with reactive organic monolayers and demonstrate functionalization of the patterned substrates with small molecules and proteins. The approach completely protects surfaces from chemical oxidation, provides precise control over feature morphology, and provides ready access to chemically discriminated patterns.

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Bioengineering

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
Graham Marsh 1, Richard E. Waugh 1
1Department of Biomedical Engineering, University of Rochester

The mechanical characteristics of endothelial glycocalyx were measured by indentation using micron sized spheres on AFM cantilevers. Endothelial cells were cultured in a custom chamber under physiological flow conditions to induce glycocalyx expression. Data were analyzed using a thin film model to determine the glycocalyx thickness and modulus.

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