University of Rochester

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium

Carleen M. Bowers¹, Eric J. Toone¹, Robert L. Clark², Alexander A. Shestopalov³

¹Department of Chemistry, Duke University , ²Hajim School of Engineering and Applied Sciences, University of Rochester , ³Department of Chemical Engineering, University of Rochester

Here we describe a simple method for patterning oxide-free silicon and germanium with reactive organic monolayers and demonstrate functionalization of the patterned substrates with small molecules and proteins. The approach completely protects surfaces from chemical oxidation, provides precise control over feature morphology, and provides ready access to chemically discriminated patterns.

Bioengineering

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy

Graham Marsh¹, Richard E. Waugh¹

¹Department of Biomedical Engineering, University of Rochester

The mechanical characteristics of endothelial glycocalyx were measured by indentation using micron sized spheres on AFM cantilevers. Endothelial cells were cultured in a custom chamber under physiological flow conditions to induce glycocalyx expression. Data were analyzed using a thin film model to determine the glycocalyx thickness and modulus.