University of Victoria

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration

Anthony Renda¹, Raad Nashmi¹

¹Department of Biology, University of Victoria

We have developed a novel technique of quantifying nicotinic acetylcholine receptor changes within subcellular regions of specific subtypes of CNS neurons to better understand the mechanisms of nicotine addiction by using a combination of approaches including fluorescent protein tagging of the receptor using the knock-in approach and spectral confocal imaging.

Bioengineering

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

Kathleen Kolehmainen¹, Stephanie M. Willerth²

¹Department of Biology, University of Victoria , ²Department of Mechanical Engineering, Division of Medical Sciences, University of Victoria

This work details the preparation of 3D fibrin scaffolds for culturing and differentiating plutipotent stem cells. Such scaffolds can be used to screen the effects of various biological compounds on stem cell behavior as well as modified to contain drug delivery systems.